The radioactive labeling of proteins with an iodinated amidination
ANALYTICAL BIOCHEMISTRY 69, 339-349 (1975) The Radioactive Labeling of Proteins with an Iodinated Amidination Reagent FREDERICK T. WOOD, ...
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Fluorine-18 Labeling of Proteins - Journal of Nuclear Medicine
18F-labeled reagents for protein labeling methyl ...... The radioactive labeling of proteins with an iodinated amidination reagent. Anal Biochem 1975; 69:339-349 ...
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Tritium Labeling of Proteins to High Specific Radioactivity by
Feb 14, 1980 ... Tritium Labeling of Proteins to High Specific Radioactivity by. Reductive ... life of iodinated protein samples is generally much shorter. * The costs of .... was required to provide a 2- to 6-fold molar excess of this reactant over total lysyl ..... Although guanidination and amidination do not affect the net charge of ...
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SUMMARY Purified parathyroid hormone can be readily tritiated by
Iodinated parathyroid hormone has been used for immuno- assays (8, 9) and ... Cm -0 --CHa + protein-NTJ? -+ .... amidination of this hormone actually bc achieved; and (c) if the foregoing are ... reaction conditions without addition of the labeling reagent it- self. ... demonstration that radioactivity is directly bound to the poly-.
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Uptake and degradation of high density lipoprotein - The Journal of
In contrast, the binding of 1251-labeled HDL to HFH cells was actually ...... radioactive labeling of proteins with an iodinated amidination reagent. Anal. Biochem ...
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Group-Specific Reagents in Protein Chemistry - Annual Reviews
environment, or (b) interaction between the reagent and the protein environ- ment, or both ... peptides by color, fluorescence, .or radioactivity; (g) the availability of rela- ... specific or affinity labeling has been omitted from this review ; bifunctional ..... posed as colored reagents for amidination, to permit visible identification and.
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Attachment of Thioglycosides to Proteins: Enhancement of Liver
Apr 19, 1976 ... BINDING OF NEOCLYCOPROTEINS TO LIVER MEMBRANE. Maekawa ... and bovine serum albumin by amidination, diazo coupling, and ... reagents which we have described in the preceding paper (Lee ... Asialoorosomucoid was iodinated .... than 5% of the radioactivity was bound in the absence of any.
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Biochemical Comparison of Pili from Variants of - Microbiology
Gonococci produce filamentous protein pili which are probably important .... The amidination reaction was allowed to proceed for. 48 h at 37 ..... radioactive labelling of proteins with an iodinated amidination reagent, Analytical Biochemistry 69,.
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The reaction of protein amino groups with methyl 5-iodopyridine-2
The extent of reaction of this reagent with protein amino groups can be readily ... for preparing heavy-atom isomorphous derivatives of proteins is discussed.
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and tyrosine in the ovarian pedicle in sheep - Reproduction
Radioactive substances were infused into an ovarian venous branch about 15-20 mm ..... radioactive labelling of proteins with an iodinated amidination reagent.
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Endocytosis of the ASGP Receptor HI Is Reduced by Mutation of
tion of fluid phase and certain toxins and membrane proteins. (reviewed by .... K. Protease-resistant labeled receptor was analyzed by immunoprecipita- tion, SDS -gel ... Asialoorosomucoid (ASOR) was prepared and1251-iodinated as described ... ligand was determined by counting the radioactivity, and by gel electropho-.
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Dec 12, 1990 ... ecules for labeling proteins, particularly antibodies, useful for clinical diagnosis ... For example, radioiodinated ferritin is used in an in ... reagent, methyl-p- hydroxybenzimidate, can be radioio .... gation conditions, such as acylation or amidination, that .... of the radioactive compound to the protein solution (or.
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Hemin Uptake in Porphyromonas gingivalis: Omp26 Is a - American
A 26-kDa outer membrane protein (Omp26) has been proposed to play a role in .... days when stored in the dark at 4°C. All reagents were ... Radioactive hemin uptake. ... Porphyrin amidination. ... the iodinated photoreactive end covalently linked to a target ... terminated by transfer of the 125I-labeled SASD solution into.
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Download as a PDF - CiteSeerX
of glycosylation on the metabolism of a naturally occurring plasma protein. In these .... iodinated using a solid phase lactoperoxidase glucose oxidase method, exactly ... manner. Protein bound radioactivity was determined by precipitation in 10% .... I-labeled amylase. A. This revealed a striking accumulation of radio- activity.
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Blood-Brain Barrier Permeability of Novel [D-Arg ] - Journal of
Dec 8, 2003 ... The total radioactivity was counted with a ... using BCA protein assay reagent ( Pierce Chemical, Rockford, IL). Effects of Metabolic ... Cell/medium (μL/mg protein) of 125I-labeled peptide ..... that N-terminal amidination of TAPA increases the potential .... us to use laboratory facilities for the radio-iodination.
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