Separation of Tubulin Isoforms by Isoelectric Focusing in
Notes & Tips. Separation of Tubulin Isoforms by Isoelectric Focusing in Immobilized pH Gradient Gels. Author links open the overlay panel. Numbers correspond ...
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Semiquantitative Analysis of Amyloid β Peptides Using a
Separation of Tubulin Isoforms by Isoelectric Focusing in Immobilized pH Gradient Gels. 1999, Analytical Biochemistry more. Robley C. Williams, Chirayu Shah, ...
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1 Two-dimensional gel electrophoresis in proteomics: a - arXiv.org
With immobilized pH gradients the reproducibility of the separations made a quantum ... order of separation is isoelectric focusing first and SDS electrophoresis second. ...... Tubulin Isoforms by 2-Dimensional Gel- Electrophoresis Using Sds- ...
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Transglutaminase and Polyamination of Tubulin: Posttranslational
Following cold/Ca2+ fractionation, samples were separated on gradient gels as ... for 30 minutes at 4°C and absorbed onto a 7 cm immobilized pH gradient pH ... containing dithiothreitol and separated by preparative isoelectric focusing ..... C) Alignments of N-termini of major beta tubulin isoforms in both the mouse and.
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Identification of β‐Tubulin Isoform V as an Autoantigen in Allergic
Specifically, we separated proteins extracted from peripheral blood ... tified autoantigens, ß-tubulin isoform V (ß-tubV), using a recombinant protein ... 2DE, 2- dimensional electrophoresis; IEF, isoelectric focusing; ... lized pH gradient (IPG) buffer (pH range 3–10; Amer- ..... using reswelling of dry immobilized pH gradients.
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Development of the Double Cyclic Peptide - ACS Publications
Jun 30, 2016 ... compared with that of the Pro-A/G-immobilized beads. The conjugate of Fc-III-4C .... eluted and separated on 1D SDS-PAGE (Figure S6). The .... Separation of tubulin isoforms by isoelectric focusing in immobilized pH gradient.
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Two-Dimensional Gel Electrophoretic Analysis of Vectorially
pH gradient electrophoresis (NEPHGE) followed by polyacryl- amide gel ... In addition, novel isoforms of actin, 1I-tubulin,. PH-20, and .... Proteins were separated by IEF/PAGE (A, C) and by NEPHGE/PAGE (B, D) resulting in ..... C) Autoradiogram of reduced proteins immobilized on nitrocellulose membrane. Horizontal ...
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Identification and Characterization of a Pi Isoform of Glutathione S
May 13, 2004 ... tubulin, high-density lipoprotein, low-density lipopro- ... transferase Pi; HDL, high- density lipoprotein; IEF, isoelectric ... (pH 7.4) containing 8 mM CHAPS, followed by a linear gradient from 0 ... Phase separation was promoted by the ... tric focusing (IEF) separations were done using precast immobilized pH.
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Proteomic Analysis of the Highly Detergent Resistant Radial
pH gradient (IPG) strip or liquid-phase isoelectric focusing (IEF) followed by .... 2.3.3 Two Dimensional Gel Electrophoresis with Immobilized pH Gradients..21 .... Figure 3.1- Flow chart summarizing the isolation, the separation technique, and ..... 21.5kDa and 17kDa isoforms of MBP, and later CNP, tubulin, actin, OSP, and ...
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Comparative Proteome Analysis of Native Differentiated and - IOVS
lular proteins were separated by isoelectric focusing using immobilized pH gradients (IPG 3–10) and electrophoresis on. 9% to 15% gradient polyacrylamide ...
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A gel-based reference map of the porcine hepatocyte proteome
Following isoelectric focusing with three pH range immobilized pH gradient strips (pH 3–6, ... and proteins were separated by two-dimensional poly- acrylamide ...
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Acetylation of ar-Tubulin in Cultured Neurons and the Induction of
by high-resolution isoelectric focusing (Marotta et al., 1978;. Received July 25, 1986 ... A similar variety of tubulin isoforms also has been ob- served in cultures of ..... with )H-acetate and separated into CBM and neurites, and the resulting ..... sional electrophoresis of tubulin using immobilized pH gradients un- der denaturing ...
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2-D Electrophoresis for Proteomics - Bio-Rad
For 2-D PAGE, IEF is best performed in an immobilized pH gradient ..... IPG strip. Second-dimension separation was in 10–24% gradient gels with PDA ..... stathmin. HSP 60 tubulin. (nonlinear). For ordering information related to this section.
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A highly epothilone B–resistant A549 cell line with mutations in
mutations in tubulin that confer drug dependence. Chia-Ping Huang .... Separation of Tubulin Isotypes by Isoelectric Focusing .... expression of mutant tubulin isoforms at the protein level. (20, 21) ... Coomassie-stained immobilized pH gradient.
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2D Differential In-gel Electrophoresis for the Identification of
are separated in the same gel, those proteins existing in both pools will migrate to ... Materials—Cy3, Cy5, immobilized pH gradient strips, Pharmalyte, and ECL ... 2D DIGE and Image Analysis—First-dimension isoelectric focusing was carried ..... Hanash, S. M. (2000) Identification of ß-tubulin isoforms as tumor anti- gens in ...
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Current Status of Two-Dimensional Gel Electrophoresis and Multi
Oct 16, 2015 ... PTMs, isoforms, spatial structure of each protein, protein-protein ... The common proteomic separation techniques include Two-Dimensional ... direction of Isoelectric Focusing (IEF) and Molecular Weight (Mw) in the direction of Sodium .... of two-dimensional electrophoresis with immobilized pH gradients.
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Parfait Sendegeya, Guan-Nan Li, Huan-Huan Zhao, Shi Bao He
immobilized pH gradients strip (IPG strip, 18 cm, line, pH 3–10, GE Healthcare. Co.) .... separated by isoelectric focusing (IEF) using 18 cm immobilized pH gradients ... subunit isoform 2, enolase, vacuole ATP synthase catalytic subunit A, vacuole .... associated with carbohydrate metabolism;7 β-tubulin which is one of the two.
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Cyclin-dependent kinases regulate splice-specific targeting of
Oct 9, 2012 ... Drp1-001 and -101 with ß-tubulin was confirmed by quantitative image analysis .... for separation of similar mass Drp1 isoforms by isoelectric fo- cusing on narrow pH .... spots toward the acidic end of the pH gradient (one of which identified as ... alkaline phosphatase before isoelectric focusing (Fig. 2 C).
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Latitudinal Variation in Protein Expression After Heat Stress in the
May 28, 2012 ... (408C). We separated gill proteins using two-dimensional gel electrophoresis and quantified .... An immobilized pH gradient. (IPG) IEF strip (11cm; pH 4–7; Biorad) was laid ..... One b-tubulin (spot 12) isoform and one a-tubulin.
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Neuroproteomics of the Synapse and Drug Addiction - Journal of
Apr 27, 2006 ... Protein profiling generally involves the separation of proteins or tryptic ... (IEF), using an immobilized pH gradient (IPG) strip. ..... Several cytoskeletal proteins ( such as various isoforms of actin and tubulin) were significantly.
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