Purification of pepsinogen I from human urine by means of DEAE
Purification of pepsinogen I from human urine by means of DEAE- chromatography and isotachophoresis. The present paper gives a description of a procedure ...
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Pepsinogens and pepsins - Gut
Under this definition, the rennins could be regarded as types of ... Since activation of pepsinogen takes place in the presence of acid and is catalyzed by pepsin ... Human urine was found to contain only pepsinogens II and III, and on activation ... of Kushner"l and the DEAE-cellulose chromatography method of Seijffers is.
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Extraction, Purification and Characterization of Fish Pepsin: A
Nov 17, 2011 ... Isolation and purification of pepsin from fish and fish wastes is less ... and can be isolated from a variety of species such as human, cattles, ... be found in limited amounts in the blood, muscle and the urine . ... pepsinogen (PG) is mainly based on (a) crude enzyme extraction by homogenization and ...
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The Fractionation of Urine Colloids on Anion-Exchange - NCBI
purification experiments were severely hampered .... geneous glycoprotein fractions from normal urine. ... from the DEAE-cellulose column, and to 0-25 ml. samples .... complete chromatographic separation was accomplished in ... urine colloids on DEAE-cellulose column .... antisera prepared in chickens to human- serum.
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Molecular Model for the Binary Complex of Uropepsin and - Unesp
The three-dimensional structure of human uropep- sin complexed with ... handling of pepsinogen A and pepsinogen C (5). Stud- ... urine have shown that the amount of pepsinogens in the urine ... The uropepsin was purified using the same procedure ... performed by a three-step procedure: DEAE Bio Gel (Bio Rad) chro-.
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electronic reprint Structure of human uropepsin at 2.45˚A resolution
proteinase from the urine produced in the form of pepsinogen. A in the ... are stabilized in an inactive conformation by means of electrostatic ... Purification of uropepsin. Human ... dure: DEAE bio gel (Biorad) chromatography, Mono Q 5/5.
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Assay of gastricsin and individual pepsins in human - Europe PMC
The mean percentage content of pepsins 3a, 3b, 3c, and 1 in non-stimulated gastric ... England), TSK DEAE 5 PW 7-5 x 0-75 cm ... Figure 1 Ion exchange chromatography offour human ..... relate the separate pepsinogens in urine,2' or ... between mucosal pepsinogen and gastric .... Pepsinogens I and II: purification from.
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Agammaglobulinemia - Cloudfront.net
The light chains of the urine constitute more than half the total gamma .... were fully absorbed withappropriate purified gamma ... Mean. 1.64. 0.35. 0.18. 1.9. Median. 1.25. 0.31. 0.16. 1.9. * Human serum ..... ISOLATED BY PREPARATIVE DEAE CHROMATOGRAPHY. ..... Separation of pepsinogen II and pepsinogen III.
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Method of producing γ-globulin for intravenous injection and
May 26, 1982 ... lytic enzyme and a stabilizer, to human 'y-globulin. Uro~ pepsinogen can be also as a stabilizer. .... tended in this speci?cation to mean both pepsin and .... trated, then further puri?ed by gel chromatography .... trated'urine were appliedto a DEAE-cellulose (What ... column (2.5X90 cm) for further purification.
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