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Construction of a chassis for hydrogen production - Microbiology
6803 is the best studied cyanobacterial strain and its genome was the first to be ... photoautotrophic chassis in synthetic biology applications. To prepare it for the introduction of a .... H2 production (McIntosh et al., 2011); however, further.
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Yu, J., Liberton, M., Cliften, P. F., Head, R. D., Jacobs, J. M., Smith
Jan 30, 2015 ... PCC 7002, have been used in synthetic biology studies for biosynthesis ... strain in order to build a superior cyanobacterial chassis for synthetic ..... sample of Synechococcus UTEX 625 was thawed in 2011 and a single colony from the .... reductant flux into hydrogen production via metabolic engineering of.
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Scaling up synthetic biology: Do not forget the chassis - ScienceDirect
Jan 2, 2012 ... General properties of the chassis required for synthetic biology are explored. .... proposed as a source for enzymes using hydrogen in a fuel cell [15], has a long genome. ... Finally, core metabolic processes, including energy production and .... Preparing synthetic DNA with the proper shape is therefore ...
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The PLOS ONE Synthetic Biology Collection: Six Years and Counting
The funders had no role in the preparation of the manuscript. Competing ... emergence of synthetic biology as a scientific community. This is .... reading frame. As well as .... 13 enzymes for high-yield hydrogen production from starch and water [35]. ... some cyanobacteria were relatively harmless in zebrafish embryos,.
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S. elongatus - ACS Publications - American Chemical Society
Apr 7, 2016 ... limiting.10 Wildtype A. vinelandii is rod-shaped and around 2 μm in length and ... To provide a photosynthetic carbon source for A. vinelandii, ... cyanobacterial model system.19,20 Unlike many cyanobacteria, such as ... engineered further for the production of energy-rich products ... ACS Synthetic Biology.
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Synthetic biology: An emerging research field in - Markus Schmidt
Jun 25, 2011 ... minimal cell factories and research on chemical synthetic biology is focused on artificial proteins and ... industrial production, and to provide health care to the 1.3 billion .... sustainable resources (hydrogen, wind, solar and biomass). ... of QIBEBT was to engineer cyanobacteria as a chassis organism for the.
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Synthetic Biology. Latest developments, biosafety considerations
Synthetic Biology (SB) is a multidisciplinary and rapidly evolving field. ... biological circuits; (ii) Defining a minimal genome/minimal life (top-down approach); (iii) Constructing ... commercial production of substances in contained facilities. ..... cyanobacterial species and eukaryotic algae has also recently been explored for ...
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China's Synthetic Biology Research - World Scientific
Recent synthetic biology efforts have led to successful production of various biofuels in engineered cyanobacteria systems, including ethanol, 1-butanol ... biodiesel, and hydrogen, demonstrating that the large-scale production of biofuels ... (3) Streptomyces chassis for the production of microbial medicines,. (4 ) Validamycin ...
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Modelling and analysis of biological systems to obtain biofuels
to efficiently obtain biofuels, such as ethanol and hydrogen. As a target ..... PhD thesis stands on: systems biology, cyanobacterial biology and energy engineering. ... Construction of a chassis for hydrogen production: physiological and molecular ... Manuscript in preparation. ... Urchueguía: Introduction to Synthetic Biology.
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RECONSTRUCTION AND ANALYSIS OF GENOME-SCALE
microbial production chassis. Here we assemble, update and .... Chapter 3 SYNTHETIC BIOLOGY OF CYANOBACTERIA: UNIQUE. CHALLENGES AND ... 3.5.2 Redirecting Carbon Flux by decoupling growth from production ..... 56 ..... 6.4.3 RNA and DNA Preparation . ...... isoprene, hydrogen and biofuels [89-97]. However ...
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Synthetic lipid-containing scaffolds enhance production by - bioRxiv
May 6, 2016 ... 1Department of Systems Biology, Harvard Medical School, 02115, Boston, ... encapsulated bacteriophage φ6 to form synthetic lipid-containing .... of foci per cell varied with the induction level, cells induced for 2 .... The eluate of the P9- 6His preparation contains P9-mCitrine fusions that can be detected by.
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Potential Impacts of Synthetic Biology - Convention on Biological
Oct 17, 2011 ... Part 2: Synthetic Biology, Biodiversity and Biosafety ... H. The Cartagena Protocol does not sufficiently cover synthetic biology and its potential ... production processes: 1) biofuels; 2) specialty and bulk chemicals; 3) natural product ...... cyanobacteria engineered to produce sugars to frame the discussion.
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Engineering Synechocystis PCC6803 for Hydrogen Production
Feb 24, 2014 ... complex cyanobacterial machine for hydrogen production (Figure. S1) is best studied in ... of the photosynthetic gene psbAII to increase the expression of the ... effective cyanobacterial factories for the biological production of hydrogen ... for in frame-fusion of the protein coding region of the studied genes [5 ...
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Quorum Sensing Communication Modules for Microbial Consortia
any singular microbe, a new frontier in synthetic biology is emerging: synthetic ecology, or .... the resulting GFP production after 3 h of incubation was measured  ...
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Reconstruction and comparison of the metabolic - Costas Maranas
Oct 31, 2012 ... microbial production chassis. Here we ... Cyanobacteria represent a widespread group of photosynthetic ... and contribute significantly to biological carbon sequestration, O2 ... capabilities including nitrogen fixation and H2 production [19 ], ... pooled. Spectra of this extract and of a sample of whole cells were.
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Developments in the tools and methodologies of synthetic biology..pdf
Nov 26, 2014 ... 2 Department of Medicine, Imperial College London, London, UK. Edited by: ... between synthetic biology and genetic engineering (Andrianan- toandro et al., 2006 ... that has some influence over chassis (host cell) choice, the design ..... Protein/metabolite production is decoupled from the need of the cell to.
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Scaling up synthetic biology: Do not forget the chassis
Jan 2, 2012 ... Introduction. The present avatar of «Synthetic Biology» (SB) assumes that we .... Some cyanobacteria, using carbon dioxide as carbon ... autotroph proposed as a source for enzymes using hydrogen in a ... abolic processes, including energy production and management (in ..... Preparing synthetic DNA with.
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Genetic Analysis of the Hox Hydrogenase in the Cyanobacterium
Jun 15, 2012 ... (6), and the purple sulfur photosynthetic bacteria Thiocapsa roseopersicina ... an electron valve for cells by H2 production/oxidation in ... frame (ORF) replacement, combining ... Cyanobacterial Hox Hydrogenase Complex Association and Function ... boiled in 1 Laemmli sample buffer (Bio-Rad), and 10 μl of.
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Programme - ECB
Jul 5, 2016 ... Carboxylate reductases from fungal origin for the preparation of ... Microbial itaconic acid production as test case for molecular cell .... Systems and synthetic biology for biocatalysis (Session 2) will be .... Chassis organism from Corynebacterium glutamicum - Genome ..... hydrogen as a new energy source.
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A Powerful Toolkit for Synthetic Biology: Over 3.8 Billion Years of
Knight(2) and Endy(3) see synthetic biology as an engineering challenge ... '' chassis'' organism would be created to provide a blank canvas upon ... Hydrogen produced during the Big Bang is interchangeable with hydrogen produced today. .... cyanobacteria. .... The rates of the production of genetic novelty can vary with.
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