Preparation of a stable folate-Sepharose complex for affinity
A stable folic acid affinity gel has been developed for the purification of nanograms .... Folate-Sepharose column as an affinity absorbent for protein fractionation.
[ 1-s2.0-0003269778902671-main.pdf?_tid=524a0f4e-f3d6-11e4-80f6-00000aacb35f&acdnat=1430906435_50bd3fa8b885f263456a29334a83f4a7 - Read/Download File
Affinity Chromatography of Dihydrofolate Reductase - NCBI
studied on affinity columns containing folic acid and methotrexate. Two forms of .... Specific activity of the enzyme is expressed in units/mg of protein. Protein was ...
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Purification of folate binding factor in normalumbilical cord serum
very high affinity binder and was present in concentrations ... cm column of AH- Sepharose 4B (Pharmacia) to which ... er until no detectable material absorbing at 280 nm was eluted ... Peak fractions of binding activity (Fig. 3) ... volume protein*.
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Purification and Characterization of GTP Cyclohydrolase I from
May 7, 1985 ... riboflavin and folic acid (6). The diverse .... daltons being the major fraction of total protein (Fig. 1). The ... Sepharose column retained GTP cyclohydrolase I activity in ..... cyclohydrolase I were centered on affinity chromatography ..... f i l t e r e d t h r o u g h 0.6 ml Of '"on-absorbent-cotton i n the bdttan.
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Effect of a Single Amino Acid Substitution on Escherichia coli
ential elution fiom a Methotrexate affinity column. The complete .... Protein concentrations were determined by the method of Lowry et al. ... Sepharose bound about 8 pmol of Methotrexate/ml of gel. Enzyme ... buffer was made 1 mM in folic acid, and the chromatography was ... to the light-absorbing properties of the ligand.
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TALON Metal Affinity Resin FAQs - Clontech
TALON resin is an immobilized metal affinity chromatography (IMAC) resin charged with cobalt ... What types of histagged proteins does TALON resin bind to? .... 10 ml), adjusted to a pH of >7, to 1 ml of resin packed in a column. ... (<10 mM), the histagged protein in the elution fractions is not affected by the metal ion transfer ...
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Purification and Properties of Escherichia coli Dihydrofolate
that includes methotrexate affinity column chromatogra- phy. Determinations of the ... Folic acid also serves as a substrate with a single pH optimum of pH 4.5. ... AH-Sepharose 49, Sephadex G-50, and Sephadex G-100 ... bromide, and proteins used as molecular weight markers ..... Fractions containing 0.65 ml were col-.
[ bi00695a006 - Read/Download File
Identification of an N‐hydroxyguanidine reducing activity of xanthine
with high affinity . In more ... protein purification from the rat spleen cytosol, we show here .... where q is the fraction of guanabenz in a guanoxabenz/guana- .... the DEAE-Sepharose® to a hydroxyapatite column, it was eluted ... The absorbency ratio A280/A450 (the ratio of ..... on Sepharose 4B/folate gel, FEBS Lett. 131 ...
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Identification of Serum and Urine Proteins Responsible for
Mar 19, 1996 ... ... the pigment (9, 18). Proteose peptone and serum (5) and folate pathway inhibitors .... (iii) In step 3, the active fraction from step 2 was passed through a 1- ml protein A-. Sepharose column (Pharmacia), but no activity was retained. ... by the Sephacryl, which acted as an affinity absorbent. More- over, the ...
[ RosaFraile_IdentificationOfSerum.pdf - Read/Download File
Overproduction of Two Antigenically Distinct - Cancer Research
major protein band corresponding to dihydrofolate reductase when mRNA ... an altered form of DHFR resulting in reduced affinity for MTX (1, ... NaCI solution); ELISA, enzyme-linked immunoabsorbent assay. ... on MTX-Sepharose columns as described previously (19, 20). Fractions ...... R. T. Regulation of folate reducÃ- ase.
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Human Hepatic Methionine Biosynthesis - Students
Presence OfhomocYsteine and a Product ofthe reaction, the protein may be released into blood following .... transferase can utilize also certain thetins instead of betaine SePharose ... Breakthrough fractions containing the major portion of the enzyme ... rose 6B column (2.6 X 90 cm) and eluted with 5 mM potassium.
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Characterization of a broad-specificity non-haem iron - Microbiology
graphic step, eluate fractions were assayed for N-demethylase (Ndm) ... Sepharose column (GE Healthcare) pre-equilibrated in KPGD buffer ... CBB5 protein (depending on purity) in 50 mM KPi buffer. The ...... Toluene dioxygenase: purification of an iron-sulfur protein by affinity ... Cellular folate binding proteins; function and.
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Endothelial Targeting of Cowpea Mosaic Virus (CPMV) via Surface
May 1, 2009 ... the CPMV binding protein as a cell-surface form of the intermediate filament vimentin. .... concanavalin A-sepharose column to remove glycoproteins, and the flow-through fraction was bound to an affinity matrix that was ...... Lee RJ, Low PS (1994) Delivery of liposomes into cultured KB cells via folate.
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Serine Hydroxymethyltransferase from Escherichia coli: Purification
calibrate the columns were rabbit serine hydroxyme- thyltransferase, muscle .... fractions containing activity were pooled, and the protein was precipitated by the ...
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Cloning, expression, purification, and characterization of zebrafish
www.elsevier.com/locate/yprep. Protein Expression and Purification 46 (2006) 212–220 ..... phy on a 0.7 · 4-cm phenyl-Sepharose column equilibrated with 30 % ...
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Characterization of small- molecule–biomacromolecule interactions
Feb 16, 2015 ... successfully applied in affinity studies between small ... protein column, and then elute the column with a ..... using proteins or other UV-absorbing components in the .... trimethoprim, and folic acid were infused at 20 nM, fluorescein at 100 nM. ..... (Actigel B Ultraflow and NHS-activated Sepharose 4 Fast.
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Download (17MB) - ORCA - Cardiff University
Ni-NTA Sepharose column ... Figure 2.3: 12% SDS-PAGE o f eluted protein fractions after Blue. 72 ... Figure 3.24: Absorption spectrum ofphotolyase fractions ..... efficiently and with low energy consumption by absorbing near UV- or blue light .... acid residues, that are responsible for the strong binding affinity of FAD to the.
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pat6689338.pdf - ShareOK
May 31, 2001 ... 2000, cancer vaccme: peptides, proteins, and DNA. Cancer ... or a peptide having a known afﬁnity to cancer cells. One or ...... matography and the fractions collected and analyzed by ... avidin and the absorbency at 500 nm is reduced. The decrease .... biotinylated proteins, antibody fragments, folic acid, and.
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Rapid detection of analytes with receptors immobilized on soluble
binding proteins such as Intrinsic Factor or Folate Binding. Protein. Receptors are ... speci?city and affinity as the analyte. ...... Q-Sepharose column (1.0><13.0 cm) using a gradient. betWeen 20 mM ... absorbing the antibody to the column and then eluting the .... The fractions containing IgG-E5 are pooled and the protein.
[ US5898005.pdf - Read/Download File
Random detection of antigens with antibodies immobilized on
Apr 12, 1994 ... binding proteins such as Intrinsic Factor or Folate Binding. Protein. Receptors ... speci?city and affinity as the analyte. In heterogeneous ...... Pooled protein fractions containing ... Q-Sepharose column (1.0><13.0 cm) using a gradient. betWeen 20 ... absorbing the antibody to the column and then eluting the.
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