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Bromodichloromethane Inhibits Human Placental Trophoblast
disrupts syncytiotrophoblast formation and inhibits CG secretion in vitro. Although other .... BDCM inhibits trophoblast morphological differentiation and. CG secretion in a ... procedure used to isolate cytotrophoblast cells from term human placentas has ..... and King, 1993) and dimethyl sulfoxide (Thirkill and Douglas,. 1997).
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Modulation of trophoblast stem cell and giant cell phenotypes
in vitro development of trophoblast cells. .... them a valuable in vitro tool for studying the process of trophoblast cell differentiation (Kamei et al., 1997,. 2002). Dimethyl sulfoxide (DMSO) is a reagent frequently ... versibly inhibited trophoblast giant cell differentiation ..... on human and rat trophoblast cells have remarkable.
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(2000) Trophoblast differentiation
In vitro differentiation of Rcho-l trophoblast cells is also ... Cell freezing and storage medium: Standard growth medium containing 10% dimethyl sulfoxide ( Sigma). 4. ... culture plate, culture medium containing serum is added to inhibit the actions of trypsin. ..... human immunodeficiency virus (HIV) inhibitor 9-(2- phosphonyl- ...
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Rcho-1 Trophoblast Stem Cells - KU School of Medicine-Wichita
to differentiate in vitro and in vivo into trophoblast giant cells. RCHO and ..... tiating cells to dimethylsulfoxide can inhibit trophoblast giant cell differentiation ..... scriptional activation of human chorionic gonadotropin-alpha gene through protein.
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Type III Interferons Produced by Human Placental Trophoblasts
Mar 15, 2016 ... Here we show that primary human trophoblast (PHT) cells, iso- .... (D) Two preparations of PHT cells were exposed to dimethyl sulfoxide (DMSO) to inhibit cell ..... Purification, characterization, and in vitro differentiation of ...
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Critical role for transcription factor AP-2о in human trophoblast
regulated during in vitro differentiation of human trophoblast cells, with 141 of 6918 genes induced and 256 ... After demonstrating that the expression plasmid inhibited the induction of hPL and ..... cells in vitro is inhibited by dimethylsulfoxide.
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LIMK1 Regulates Human Trophoblast Invasion/Differentiation and Is
Oct 13, 2014 ... Interestingly, in humans, LIMK1 activity promotes tumor cell invasion by ..... 1 μL/ mL 10 μmol/L BMS-5 or 1 μL/mL dimethyl sulfoxide was added. .... that LIMK inhibition in vitro impairs CTB differentiation along the invasive ...
[ 1-s2.0-S0002944014005008-main.pdf?_tid=f44681ee-5843-11e4-bea5-00000aacb35d&acdnat=1413801141_e883c007ab61e9670dc95c11c7db3706 - Read/Download File

Cyclosporin A Promotes Growth and Invasiveness In Vitro of Human
Mar 5, 2008 ... Trophoblast Cells Via MAPK3/MAPK1-Mediated AP1 and Ca. 2+. /Calcineurin/ ... could inhibit apoptosis of human first-trimester trophoblasts. However ... differentiation, tissue remodeling, migration, and invasion. In human ... addition of dimethyl sulfoxide, which dissolved the formazan crystal. Absorbency ...
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PDF (2 MB) - American Journal of Pathology, The
the use of mouse trophoblast stem cells as a discovery platform for human placentation disorders and suggest that ..... 1 mL/mL 10 mmol/L BMS-5 or 1 mL/ mL dimethyl sulfoxide .... LIMK inhibition blocks CTB invasion and differentiation in vitro.
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Adhesive and Degradative Properties of Human Placental
Placental Cytotrophoblast Cells In Vitro. Susan J. Fisher ... human trophoblast cells to penetrate the uterus are unique. ... inhibit rabbit blastocyst attachment, and Glass et al. .... on differentiated epithelia but not on connective tissue, endothelia, muscle, .... dimethyl sulfoxide (e.g., p-nltrophenyl-p'-guanidobenzoate ) or ethanol.
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The human immunodeficiency virus (HIV) inhibitor 9‐(2
posed to dimethylsulfoxide (DMSO) and other compounds such as retinoic ... model for studying cell differentiation in vitro (Schwartz et al.,. 1992). These cells  ...
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Binding of galectin-1 (gal-1) on trophoblast cells and inhibition of
Jun 8, 2004 ... BeWo cells in contrast to Jeg3 form a syncytium in vitro and ... 40 μl 10 mM biotinyl-N-hydroxysuccinimide in dimethylsulfoxide (Boorsma et al. ..... The cell lines Jeg3 and BeWo are trophoblastic tumor cells at various stages of differentiation. .... mucin 1 in the human placenta and in trophoblast cells in vitro.
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Biological actions of drug solvents
Key words: dimethylsulfoxide, propylene .... ted differentiation of human trophoblast cells and pro- ..... trophoblast cells in vitro is inhibited by dimethylsulfoxide.
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Placental lactogen-I gene activation in differentiating trophoblast
expressed as trophoblast giant cells differentiate. In this report, we ... factors that inhibit PL-I production or modulate PL-I ... Rcho-1 trophoblast cells represent an in vitro rat tro- phoblast ..... gene expression by various lineages of human trophoblast cells .... dimethylsulfoxide (DMSO) vehicle control for another 3 h. Growth.
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Quantifying the syncytialisation of human placental trophoblast
Keywords: Trophoblast; Syncytialisation; Cell fusion; FACS; BeWo cell. 1. Introduction ... differentiate into syncytiotrophoblasts which are likely to be polarised by forskolin [9,10] ... vehicle (dimethyl sulfoxide), followed by further incubation for the indicated .... entiation process in human cytotrophoblast, in vitro , J. Cell. Physiol.
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Phosphatidylinositol 3 kinase modulation of trophoblast cell
Upon differentiation of trophoblast cells, PI3K is activated leading to the ... vitro model to gain a better understanding of trophoblast cell differentiation. ... final concentration of dimethyl sulfoxide, DMSO) or Differentiation Medium supplemented with LY294002 (10 μM). ...... In human trophoblast, S1P inhibits differentiation [42].
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Notch Signaling Plays a Critical Role in Motility and Differentiation of
Nov 4, 2013 ... Failures in human extravillous trophoblast (EVT) development could be involved in the pathogen- ... Notch inhibition also increased motility of SGHPL-5 cells through .... chem) or dimethylsulfoxide (DMSO 1:1000; Sigma) as a control ...... characterization, and in vitro differentiation of cytotrophoblasts from hu ...
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Vascular Endothelial Growth Factor-A (VEGF-A) - Endocrine Society
Aug 20, 2015 ... inhibitor SB431542 abolished the stimulatory effects of activin A on ... has previously been modeled in vitro by culturing EVT ... Aberrant EVT endovascular differentiation leading to im- ... dimethylsulfoxide; EVT, extravillous cytotrophoblast; FBS, fetal bo- ... to be expressed in human trophoblast cells (21).
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The p38 Mitogen-Activated Protein Kinase - Endocrine Society
Jun 4, 2009 ... (11 -HSD2) Expression in Human Trophoblast Cells through .... differentiation because its inhibition with a pharmacological inhibitor ... vitro model system. Materials and ... lent volume of vehicle (ethanol or dimethylsulfoxide).
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Acteoside inhibits human promyelocytic HL-60 leukemia cell
Jul 18, 2007 ... tion, cell cycle regulation and differentiation of HL-60 human promyelocytic .... dependent kinase inhibitor; DMSO, dimethyl sulfoxide; DTT, dithiothreitol; ... The in vitro growth inhibition effect of acteoside on the HL-60 cells was de- ...... regulate human trophoblastic differentiation: mechanistic insights. Mol.
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