Development of a sensitive and group‐specific polyclonal antibody
An LMG polyclonal antibody-based enzyme-linked immunosorbent assay (ELISA ) was developed .... LMG and MG and to develop a sensitive and group-specific.
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Development of a Quantitative and Sensitive Enzyme-Linked
Development of a Quantitative and Sensitive Enzyme-Linked ... belonging to the group of molecules known as mycotoxins, is known to be ... polyclonal antibodies , IgY. Although the ... specific polyclonal antibodies from the yolk of a laying hen and the .... A "simplified" methanol-based extraction procedure was evaluated and ...
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Development of a sensitive and specific enzyme-linked
Sep 15, 2014 ... A competitive enzyme-linked immunosorbent assay (cELISA) has been developed and validated ... with previous polyclonal antibodies. ... determination of udioxonil in food are mostly based on .... Two groups of four BALB/c.
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Monoclonal-Antibody-Based Enzyme-Linked Immunosorbent Assay
May 23, 1988 ... an enzyme-linked immunosorbent assay (ELISA) for the detection of T. vaginalis directly from vaginal exudates. The ELISA employs ... A polyclonal rabbit anti-T. vaginalis antibody labeled ... Clearly, a need exists for a rapid, specific, and sensitive ... to the development of the enzyme-linked immunosorbent.
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Development of a sensitive monoclonalantibody-based enzyme
Development of a sensitive monoclonalantibody-based enzyme-linked ... to develop a highly sensitive and specific indirect competitive enzyme-linked ... were based on either polyclonal antibodies (pAbs) or monoclonal antibodies ( mAbs). ..... The artemisinin molecule lacking a reactive group is difficult to covalently link to a ...
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Development of chemiluminescent enzyme immunoassay for the
Jan 13, 2014 ... The CL immunoassay is a combination of sensitive CL detection and specific ..... group-specific polyclonal antibody-based enzyme-linked ...
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Development of an enzyme linked immunosorbant assay for the
Apr 28, 2011 ... Key words: Cry2Ab, enzymelinked immunosorbant assay, limit of detection, monoclonal antibody, ... Figure 2. protein gel blot analysis to specify the polyclonal antibody ... In the present study, we have developed a sensitive and specific. MAb based sandwich ELISA for the detection of Cry2Ab in GM.
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Development of an Enzyme-Linked Immunosorbent Assay for the
A competitive enzyme-linked immunosorbent assay (ELISA) was developed for the ... range of insects.5 The most sensitive and toxicologically ... thiophosphate group, which has been used successfully in ... which specific polyclonal antibodies to bromophos were ..... Based on the relatively high Amax value and slope of the.
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Development of an ELISA for the Detection of the Residues of the
A competitive enzyme-linked immunosorbent assay (ELISA) for the chloronicotinyl insecticide imidacloprid was developed using a polyclonal antibody produced against a hapten ... The high cross-reactivity with a metabolite containing the carbonyl group in the ... concentration, and pH on the sensitivity were evaluated.
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Enhanced Sensitive Immunoassay - Hammock Laboratory of
Jul 31, 2014 ... An anti-LMG monoclonal antibody (mAb) was used to select immunocomplex binding ... chemically synthesized competing hapten-based enzyme-linked ..... Deng, A. Development of a sensitive and group-specific polyclonal.
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Development of an Enzyme-Linked Immunosorbent Assay Method
Dec 28, 2015 ... Our results showed that the monoclonal antibody 2 G6-based CI-ELISA ... Keywords: aflatoxin G1; class-specific; monoclonal antibody; ELISA; Aspergillus flavus. 1. ... Production of polyclonal antibodies is simple and easy when ... B- group aflatoxins; it had a sensitivity comparable to that reported previously ...
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An Immunoassay for Dibutyl Phthalate Based on Direct Hapten
Dec 27, 2011 ... Methodology/Principal Findings: A monoclonal antibody specific to DBP was ... An indirect competitive enzyme-linked immunosorbent assay (icELISA) ... of DBP, was covalently linked to the carboxyl groups of polystyrene surface with 1- ... assay (ELISA) for phthalates also based on polyclonal antibody. .
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Development of an enzyme-linked immunosorbent assay for
Development of an enzyme-linked immunosorbent assay for screening ... haptens were synthesized and conjugated to immunizing proteins to raise rabbit polyclonal antibodies. The antibody-coated format (direct) gave better sensitivity than the ... four different potential immunizing haptens and based on these.
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An enzyme-linked immunosorbent assay (ELISA) for the major
A sandwich-ELISA based on the rabbit antibodies takes less than 2 h to ... another two groups confirmed that tropomyosin is the major heat-stable shrimp ... The assay has a sensitive detection limit of about 1 ppm and takes less than 2 h to ..... described the development of a polyclonal antibody-based sandwich ELISA that ...
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jmm.sgmjournals.org - Journal of Medical Microbiology
A mAb-based simple, specific and rapid two-tip dipstick ELISA was ... polyclonal antibodies and murine mAbs were raised against recombinant ... therefore, to have anti-V. cholerae group-specific anti- .... For the development of the two-tip dipstick ELISA ..... cholera toxin by a highly sensitive bead-enzyme linked immuno-.
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Preparation of high-affinity rabbit monoclonal antibodies for
ciprofloxacin and development of an indirect ... 9, 2010. Abstract: A convenient competitive enzyme-linked immunosorbent assay (ELISA) for ciprofloxacin ( CPFX) was ... Fluoroquinolone antibiotics (FQs) are a group of ... methods are sensitive and highly specific, but they ... ported that the LOD of polyclonal CPFX antibody.
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Enzyme-linked immunosorbent assay for human plasma
Abstract A noncompetitive enzyme-linked immunosorbent assay (ELISA) has been ... ranges for plasma apoB levels based on 1115 subjects are presented.
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Development of radioimmunometric assays and kits for non-clinical
Production of antibodies against aflatoxin B1 and development of aflatoxin B1 ELISA . .... The utility of RIA and enzyme immunoassays for environmental .... One group developed a specific, sensitive and accurate method for the quantitative ... Standards were based on the addition of chemically pure progesterone (Sigma or ...
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Development of An Enzyme-Linked Immunosorbent Assay for
Dec 29, 2011 ... Methods Polyclonal and monoclonal antibodies were produced in this study. ... specific competitive direct enzyme-linked immunosorbent assay (cdELISA) ... are a group of synthetic broad-spectrum ... based on derivatization with 2- nitrobenzaldehyde ... sensitive, and inexpensive screening alternative for.
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Development of Dot‐ELISA for the detection of human rotavirus
based on the detection of the common group antigen by enzyme linked immunosorbent assay (ELISA), or on the observation of rotavirus particles by electron ...
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