Determination of nucleic acids with concentrated H2SO4: II
Simultaneous determination of ribo- and deoxyribonucleic acid ... After reaction with concentrated H2SO4, DNA is determined from the absorption at 470 mμ and ...
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Method for the simultaneous determination of free/protein - sfrbm
MDA can be measured in DNA by monoclonal antibodies ... 5 mM H2SO4. ... 2. Reaction mechanisms of the assays used for the determination of (A) LOOH/ PrOOH and (B) .... Hydrochloric acid, HCl (concentrated 37%, or 12 M) (Fluka, Cat. No.
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iD PYRTMIDINE COMPONESTS OF RIROT\'UCLEIC ACID* The
As t.he pyrimidine nucleotidcs were determined from the ultra- violet absorption of the ... methods were applied to the same nucleic acid hydrolysat,e. The recent .... addition of concentrated sulfuric acid to a suspension of the picrate in .... TABLE. II. Per Cent Recovery of Purines under Various Conditions. Adenine. Guanine.
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Simultaneous Determination of Adenine and Thymine in Presence
Apr 1, 2013 ... individual and simultaneous determination of DNA bases adenine ... 2 µM to 230 µM for Ade, 100 µM to 2.3 mM for Thy and 10 µM to 0.2 mM for Gu respectively. ... could be performed in a phosphate buffer or H2SO4 solution.
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Ultraviolet Spectrophotometric Determination of Vanadium
studied hy NcCabe (2). Several ... region for the simultaneous spectrophotometric determination of vanadium, titanium ... ml. of concentrated sulfuric acid and diluting to 500 ml. with re- distilled .... mination of pentose nucleic acids in tissues. Al-.
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Microgram and Submicrogram Determination of - ACS Publications
nitrogen per 50 ml. permits the successful determination of nitrites in samples ... biochemical and possibly clinical interest, including nucleic acids ... simultaneous samples is presented here. ... 2 my, making use of sealed tube digestion with sulfuric acid ..... very small volume of a more concentrated reagent was technically.
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Aminomalonate as an Enzyme Inhibitor - NCBI
Extract II gave a positive reaction, but only when heated, with Ehrlich's reagent ... Simultaneous determination of nucleic acid and protein during thecourse of ...
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Microbial Metabolism - Applied and Environmental Microbiology
countered in determining the sources of the in vivo isotopic discriminations are ... pH 2 with concentrated HCl and extracted with chloroform to yield the fatty ... The nucleic acid fraction was prepared by a modification ... N H2SO4) to yield the purified citrate. ..... simultaneous determination of carbon, hydrogen and nitrogen.
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Intracellular Distribution of Radioactivity in Nucleic Acid tration of
comparison of nucleic acid and protein synthesis in normal and neoplastic ..... over sulfuric acid. This material was used for the determination of the C'@specific .
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A 3D Microfluidic Chip for Electrochemical Detection of - MDPI
Jan 22, 2015 ... detection of metal ions, proteins, DNA mutations and other compounds . ... (1 g) and KMnO4 (6 g) were added with stirring to concentrated H2SO4 (96%, w/w, 46 mL) ... potential 1.5 V for adenine and 1.8 V for simultaneous detection of .... 1 Coefficients of determination; 2 Limits of detection of detector (3 ...
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Determination of the Viability of Aeromonas hydrophila in Different
Apr 15, 2005 ... Anna Pianetti,1* Tania Falcioni,2 Francesca Bruscolini,1 Luigia Sabatini,1 Elivio Sisti,4 .... in concentrated H2SO4 and rinsed in hot tap water, in a 10% HNO3 solution, and ... by using the nucleic acid double-staining procedure of Barbesti et al .... based on simultaneous use of permeable ffuorescent probes.
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Quantitative detection of 4-hydroxyequilenin–DNA adducts in
Apr 20, 2010 ... long-term HRT increases the incidence of breast (2–4), ovarian (5,6) and .... antibody-rich supernatants were concentrated by ... 2M H2SO4, the absorbance of colored products derived ... determine very low levels of 4-OHEN–DNA adducts, a ..... Nikaido,O. (1991) Simultaneous establishment of monoclonal.
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X-ray crystallographic study of DNA duplex cross - Nucleic Acids
Dec 1, 2006 ... linking: simultaneous binding to two d(CGTACG)2 molecules by ... study, whilst not resulting in a full structure determination, .... excess concentrated sulphuric acid to give 9(10H)acridone- .... H2SO4, 100 C, 3 h, 99% yield. (ii) ...
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Basic UV/Visible Spectrophotometry - Biochrom
Basic UV/Visible Spectrophotometry. CONTENTS. 1. INTRODUCTION. 2. 2. ... inorganic compounds in a wide range of products and processes - in nucleic acids and ..... To determine the factor, measure the absorbance of a standard solution of known ..... set of simultaneous equations. ... Dilute sulphuric acid (0.1 N) H2SO4.
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PDF(354K) - Wiley Online Library
cation as well as of nucleic acid and protein biosynthesis diminish, with a ... Faculty, consists of the determination of glycogen content in bacteria grown in ... about four hours, with the simultaneous participation of no more than 25 students ..... N/mi: ammonium sulfate 0.035 M, brought to pH 2 with concentrated sulfuric acid:.
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Full-Text PDF - MDPI.com
Mar 3, 2016 ... Guanglu Liu 1, Yuan Wang 2, Yang Hu 1, Xiaobo Yu 2, Bin Zhu 2 and ... and expression efficiency of plasmid DNA (pEGFP-vp5) in ... are important factors that determine the transfection efficiency of plasmid DNA. ..... metallic catalyst and then suspended in a concentrated mixture acid (H2SO4:HNO3 = 3:1, ...
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TWO PHOTOMETRIC METHODS FOR THE DETERMINATION OF
Determination of Chromium in Serum Pool II by the Diphenylcarbazide ..... stabilization of tertiary structures in proteins on nucleic acids. The free chromium( III) ...
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Full Text - IAEA Publications - International Atomic Energy Agency
2. BACKGROUND INFORMATION RELATING TO THE OBJECTIVES OF THIS .... Hydrolysis of samples for the determination of adenine and guanine contents ...... by ruminants: effect of microbial nucleic acid infusion on purine derivative excretion by steers. J. .... HC1 - 0.5N (41.85 ml reagent grade concentrated HC1 / L).
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Quantitative Determination of the Biodegradable Polymer - Camo
Department of Chemistry1 and Department of Microbiology,2 Monash ... the number of methylene units together determine the identity ... simultaneous chemical analysis. ... stituents, such as proteins, lipids, polysaccharides, nucleic acids .... benzoic acid per cm3 as an internal standard and 0.15 ml of concentrated sulfuric.
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THE EFFECT OF CORTISONE ON DNA CONTENT OF RAT
DNA synthesis is a concomitant of cell division the effect of oncolytic agents on this aspect of .... In selected experiments, isolated "purified nuclei" were suspended in 2 per cent citric acid ... term DNA to indicate deoxyribose since this was the material determined. ... Oxidation was completed with concentrated nitric acid and.
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