Construction of Recombinant Escherichia coli Catalysts which
Construction of Recombinant Escherichia coli Catalysts which. Simultaneously Express an (S)-Oxynitrilase and Different. Nitrilase Variants for the Synthesis of ...
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Construction and expression of functional multi‐domain
Construction and expression of functional multi-domain polypeptides in Escherichia coli: expression of the Neurospora ... relative to non-recombinant cells. The use of this strategy for ..... Chen and Wilson (1997) recently reported the simultaneous expression of a ... target molecules or tailored catalytic activity may allow the.
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Construction and Application of Variants of the Pseudomonas
Feb 8, 2010 ... acid residue in direct proximity to the catalytic active cysteine ... Construction of recombinant E. coli strains that simultaneously express nitrilase ...
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Conversion of Sterically Demanding , -Disubstituted
May 24, 2011 ... Recombinant whole-cell catalysts that simultaneously produced the nitrilase from P. fluorescens EBC191 .... Construction of nitrilase mutants. The amino acid ... tiomers, acetophenone plus cyanide were converted by E. coli.
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Construction of Recombinant Escherichia coli JM109/LBH-10 and
Mar 21, 2014 ... by a recombinant E. coli JM109 were investigated using response surface methodology .... composed of two discrete domains in the following order: a catalytic domain (Gly-31 through .... The simultaneous effect of rice bran ...
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Download PDF - Springer
efficiency of catalysis by increasing the reaction rate in ... were found to improve the catalytic reaction. The inves- ... Recombinant E. coli expressing a novel nitrilase gene ..... van Rantwijk F, Stolz A (2009) Construction of recombinant Escherichia coli catalysts which simultaneously express an (S)-oxynitrilase and different.
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Construction and Characterization of a Single-Chain Catalytic - jstor
(single-chain antigen-binding protein/amide hydrolysis/Escherichia coli expression/antibody cloning/single-chain Fv) ... An SCA protein is a recombinant protein composed of a VL ... duce simultaneously an Aat II site at position 1 and a HindIII.
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Recombinant DNA technology and molecular cloning - Wiley
E. coli host cells, the phage λ genome circularizes by joining of the cohesive .... construction of recombinant DNA “designer weapons. ... The restriction endonuclease is loosely bound and its catalytic center is kept ... the presence of the essential cofactor Mg2+, the enzyme cleaves the DNA on both strands at the same time.
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High cell density cultivation of Escherichia coli with surface
Escherichia coli Surface-display Transglucosidase Induction Fed-batch .... The effect of lactose concentration on the catalytic activity of recombinant E. coli displaying .... In our previous work on the construction of recombinant E. coli displaying ... culture by five and seven pulses of lactose addition at the same time interval.
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Activation by Fusion of the Glutaminase and Synthetase Subunits of
Escherichia coli carbamyl-phosphate synthetase con- sists of two .... Construction of the Recombinant Plasmids—Overlap extension by polymerase chain ...
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May 21, 2009 ... expressed on the cell surface of Escherichia coli. The native outer ... can be applied not only for the generation of custom catalysts for ... Library construction through targeted mutagenesis14 generally ... Using simultaneous selection/ counter-selection screening enables .... recombinant expression in E. coli.
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Synthesis of Unnatural Flavonoids and Stilbenes by Exploiting the
Jun 22, 2007 ... Incubation of the recombinant E. coli with exogenously supplied carboxylic acids led .... Construction of Unnatural Polyketide Production System ... origins and selectable markers and simultaneously introduced in E. coli ( Figure 1B). ..... Recently discovered type III PKSs that possess novel catalytic activities, ...
[ 1-s2.0-S1074552107001500-main.pdf?_tid=d209d034-5746-11e4-b63c-00000aab0f02&acdnat=1413692421_2fe620ccd81596a66f2e35b810c9af10 - Read/Download File
Download PDF - Plos
production in Escherichia coli of the recombinant form of a cytoplasmic, .... Maturation pathway for the [NiFe] catalytic site of P. furiosus SHI is based on that proposed for E. coli hydrogenase 3 . .... Plasmid construction for rSHI production in E. coli. ..... possible to transform all four plasmids simultaneously into E. coli.
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1,3-Propanediol production by Escherichia coli using genes from
pression construct contained dhaBCE genes encoding the three subunits of ... recombinant E. coli bacteria produced 1.53 g/L of 1,3-. PDO, using 21.2 g/L of ...
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Construction and characterization of a single-chain catalytic antibody
Jan 28, 1991 ... This protein has been expressed in Escherichia coil and exhibits the same ... An SCA protein is a recombinant protein composed of a VL chain tethered to a VH ... duce simultaneously an Aat II site at position 1 and a HindIII.
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The precursor tRNA 39-CCA interaction with Escherichia coli RNase
Oct 24, 2006 ... Escherichia coli RNase P RNA is essential for catalysis by RNase P in .... the construction of a novel E. coli rnpB mutant strain. (named BW), in ...
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Assembly of lipase and P450 fatty acid decarboxylase to constitute a
Feb 26, 2015 ... Lipase P450 fatty acid decarboxylase Alkenes Cell-free catalysis Whole cell catalysis .... Production of 1-alkenes mediated by mixed resting E. coli recombinant cells .... in situ biodiesel production that occurs simultaneously with lipase .... of OleTJE and Tll. For construction of engineered E. coli strains with ...
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Synthesis of optically active amino acids from alpha-keto acids with
acids to D amino acids was also examined with recombinant E. coli cells ... system for simultaneous coenzyme regeneration (Fig. 1) has .... Construction of plasmids for the production of L and D .... We used resting cells of E. coli as the catalyst.
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UPS/pUNI Reference - SIGnAL
Nov 19, 1998 ... construction of recombinant DNA without restriction enzymes. Qinghua ... homologous recombination in Escherichia coli. ...... dozens of constructs can be made simultaneously by ..... phosphatase type 1 catalytic subunit.
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Full Article (PDF) - World Journal of Gastroenterology
Feb 21, 2005 ... Construction of recombinant E. coli was carried out as previously .... At the same time, recombinant E. coli Origami[pQE-. HBscFv] was cultured ...
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