Noninvasive Assessment of Collagen Gel Microstructure and
Multiphoton microscopy of collagen hydrogels produces second harmonic generation ... Multiphoton microscopy and scanning electron microscopy reveal that as .... Each SEM image had similar contrast and brightness, as well as the same .... The SHG and TPF signals were false-colored blue (for SHG) and green ( for TPF) ...
[ 1-s2.0-S000634950771024X-main.pdf?_tid=0cecfb02-1621-11e4-8208-00000aacb35f&acdnat=1406529373_f8007de837bb5cf982b446e8fa291a97 - Read/Download File
Noninvasive Assessment of Collagen Gel Microstructure - CiteSeerX
ABSTRACT Multiphoton microscopy of collagen hydrogels produces second harmonic ... microscopy and scanning electron microscopy reveal that as polymerization temperature ..... ments, X is the arithmetic mean fiber diameter from SEM images, and s is the ... were false-colored blue (for SHG) and green ( for TPF) and.
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New technology for assessing microstructural components of - NCBI
Feb 5, 2003 ... collagen is an important indicator of structural stability and pathologic state .... The data is displayed as a false color or gray-scale image, which represents .... scanning electron microscopy (SEM) and histology, and with higher ...
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Using transmission electron microscopy and 3View to determine
Jun 27, 2013 ... Electron microscopy of individual collagen fibrils ... here shows how 3View can be used to image the 3D organization of ...... step-through movie that is playable by QuickTime; and generate a false-color 3D reconstruction.
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[PDF] from rsc.org - RSC Publishing - Royal Society of Chemistry
Jan 11, 2016 ... microscopy IR s-SNOM, at 20 nm spatial resolution. .... IR light. The FTIR PCI false colour images were created from the ratio of amide II to amide I .... The SEM images of collagen brils before and a er tendon overload, and.
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PDF(1334K) - Wiley Online Library
Feb 28, 2003 ... scanning and transmission electron microscopy (SEM and .... were exported with false color to represent height. ... Atomic force microscopy image of (a) epineurial collagen from a control BioBreeding rat sciatic nerve and (b) ...
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Tracking Collagen Fibres through Image Volumes from SBFSEM
Serial block-face scanning electron microscopy (SBF-SEM) has become an ... image, and that false positive matches can be eliminated using a random ... dates after removing false positives using RF classifiers (right) (Best viewed in colour).
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Download PDF - Plos
Mar 13, 2014 ... beam/scanning electron microscopy (FIB/SEM) to study distribution and assembly of collagen. ... seeding density (1e7 MSC/ml) showed type II collagen molecules ..... confocal scanner with bandpass filter set to 410–450 nm. Images .... Figure 3, where the SHG signal is shown as white (false color) and.
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misra_physicochemical.pdf - KU ScholarWorks
ences in mineral/matrix ratio, crystallinity, and collagen organization were noted in the ... to permit the creation of a ''false-color composite'' image. In this study, FTIRI ..... emission-scanning electron microscopy.29,37 These comple- mentary ...
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Metabolic Dysregulation and Adipose Tissue Fibrosis: Role of
Aug 15, 2008 ... Collagen VI-null mice were generated as previously described .... scanned with the LI-COR Odyssey infrared imaging system and band intensities ..... (C) False- colored scanning electron micrographs of adipocytes from ...
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Multi-step pericellular proteolysis controls the - Stack Scientific
collagen degradation by recombinant MT1-MMP but not by trypsin. Full- ... Scanning electron micrograph and zones (numbered): 1, .... colour-coded lines in e. ..... as pixel count from thresholded images (insets, green false colour) from.
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Multimodal Highlighting of Structural Abnormalities in Diabetic Rat
Feb 25, 2014 ... also characterized by transmission electron microscopy (TEM) and second harmonic generation ... imaging corneal structures with different contrast ... tion of fibrillar collagen structures with micrometric ..... (D) False color.
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Subcellular-resolution molecular imaging within living tissue by fiber
microscopy and molecular-targeted optical labeling are two rapidly developing ... Giniunas L, Juškaitis R, Shatalin S V, “Scanning fiber-optic microscope” Electron Lett. ..... (b) False-color composite image of a 3-D collagen construct containing.
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The elastin network: its relationship with collagen and cells in
Oct 1, 2009 ... reveal the collagen matrix and the collagen fibre orientations were determined from their SHG polarization ... fibrils of 10 nm in diameter under an electron microscope. (Montes, 1996). .... transmission of the filters for the TPF detection was centred at ..... (D) CARS image of the pair of cells in false colours to.
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Automatic analysis of collagen fiber orientation in the outermost
The method uses light microscopic images of thinly sliced tissue samples for data ... Soft tissue Collagen fibers Fibrils Fiber orientation Microscopic images ...
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Ptychographic X-ray nanotomography quantifies mineral
Mar 20, 2015 ... nanofibrillar collagen is reinforced by apatite mineral crystals. ... electron microscopy imaging (BSE)9 and X-ray absorption microCT10. The former method ... We measured human dentine specimens by scanning thin (30–. 50 mm) .... Panels (c) to (e) show false-color 3D renderings of the same volume.
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Fundamentals and Abnormal Amniocentesis
B. Collagen IV forms long thick fibrils that lack a visible banding pattern by electron microscopy .... microscope. D. A scanning electron microscope produces full-color images, while ..... Identify the FALSE statement about mitochondrial DNA:.
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Characterization of Biofilm Formation by - University of New Haven
Oct 24, 2012 ... spirochetes based on observations of microscopic images of .... surfaces are coated with different matrices (collagen, fibronectin ... AFM scan made in contact mode of a pair of Borrelia burgdorferi ... Photoshop and false-color hand-painted with 3D painting tools .... AFM instead of electron microscopy.
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Strain Stiffening of Fibrillar Collagen during - ACS Publications
Apr 18, 2016 ... KEYWORDS: strain stiffening, collagen, cell migration, AFM nanoindentation, Young's modulus .... spacers (Electron Microscopy Sciences) in 24-well plates, respectively. .... imaging was performed in an Olympus FV1000 scanner (60×/ 1.35 ... left panels, overview; right panels, close-up in false color (after.
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Deep tissue imaging using spectroscopic analysis of multiply
Aug 13, 2014 ... Scattering limits the penetration depth of most optical imaging techniques. Efforts to overcome ... single time with tissue such as confocal microscopy and optical coherence .... spectroscopic information, we created a false color image based ... collagen architecture associated with the burn is also visible in.
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