Automated Analysis of Cellular Metabolites at Nanomolar to
May 25, 2002 ... Automated Analysis of Cellular Metabolites at Nanomolar to Micromolar Concentrations Using Bioluminescent Methods. P.G. Arthur,; P.W. ...
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Bioluminescent Methods for Determining Metabolites in Micro - edoc
Furthermore, the technique has been automated for the Dynatech ML2250 ... decreased the volume of sample required for the analysis ..... Automated analysis of cellular me- tabolites at nanomolar to micromolar concentrations using bio-.
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Profiling metabolites and peptides in single cells
Mar 30, 2011 ... low-micromolar levels of these two compounds imply millimolar amounts in a .... will be present at about a nanomolar concentration, so that measurements below this .... empower single-cell analysis by effecting selective gene knock- out in a ...... microfluidic device for automated single cell analysis using.
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Performance and metabolism in repeated sprint exercise: effect of
The pre- and post-test muscle metabolite concentrations, as well as the delta change .... Automated analysis of cellular metabolites at nanomolar to micromolar ...
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Cell-Based Assays - Sigma-Aldrich
drug metabolite analysis in biological fluids. Technical ... of living cells from cell signaling, proliferation, apoptosis ... an automated assay. Another ..... levels of apoptosis underlie the depletion of ..... 50–200 micromolar .... 10-500 nanomolar.
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Hypoxia Causes Downregulation of Protein and RNA Synthesis in
Abstract. 21. Arthur PG, Hochachka PW. Automated analysis of cellular metabolites at nanomolar to micromolar concentrations using bioluminescent methods.
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Determination of nitrate and phosphate in seawater at nanomolar
able for the shipboard analysis of nanomolar (nM) nitrate and phosphate ... Keywords: Liquid-waveguide-capillary cell; Nanomolar concentration; Nitrate; Nutrient;. Ocean; Phosphate ... metabolism and reproduction. Nitrogen is ... trations increase to micromolar concen- ... (2) automated methods, which are usually based on.
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CRAB Reader: A Tool for Analysis and Visualization of
automatic analysis of the information category of each sentence is therefore ..... all of these biological effects require metabolism to inorganic AsIII (O'Connor et al . ... of in vivo and in vitro models, nanomolar or low micromolar concentrations of ... however, the cellular effects of roxarsone to mammalian cells are not known.
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Download PDF - Plos
Dec 17, 2012 ... concomitantly with those of intramuscular concentrations of ...... Arthur PG, Hochachka PW (1995) Automated analysis of cellular metabolites at nanomolar to micromolar concentrations using bioluminescent methods. Anal.
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Toward Merging Untargeted and Targeted - ACS Publications
Dec 4, 2015 ... have led to discoveries in regulation of cellular metabolism by using ... micromolar range, use of MS permits detection of up to pico- and nanomolar concentrations.4 Mass spectrometry is also easily linked ... chromatography−MS tool for analysis of both polar and non- .... Recently, automated solvent-based.
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David Bishop, Johann Edge, Claire Thomas and Jacques - Cefid
Nov 2, 2006 ... Automated analysis of cellular metabolites at nanomolar to micromolar concentrations using bioluminescent methods. Anal Biochem 227: ...
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Quantitative imaging for discovery and assembly of the metabo
In vivo analysis of metabolite flux in mammalian cells. 288. XV. ... measuring metabolites with cellular and subcellular precision is a major roadblock. New tools ... and vacuolar ion and metabolite concentrations with a time resolution of milliseconds to hours. ...... covers the low nanomolar to high millimolar range completely.
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Primary cortical neuronal cultures reduce cellular energy utilization
utilizing chemical inhibition of cellular metabolism. ATP .... t-test or ANOVA with Fisher analysis. ..... Arthur P. G. and Hochachka P. W. (1995) Automated anaylsis of cellular metabolites at nanomolar to micromolar concentrations using.
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Auxin transport at cellular level: new insights supported by
Mar 20, 2012 ... nanomolar concentrations (Davies, 2010). A fast and tight ... cesses: auxin metabolism, and its transport within and .... potential of mathematical analysis of the accumulation data ... scintillation counting with automatic correction for quenching ..... carriers do not exhibit saturating behaviour until micromolar.
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Full Text (PDF) - Proceedings of the National Academy of Sciences
Jun 14, 2005 ... monitoring of glutamate metabolism in living cells, in tissues, or in ... mutant of FLIPE (with a Kd of 10 μM) expressed on the cell surface responded to perfusion with ..... covering a range of glutamate from nanomolar to millimolar. The sensors can be used for in vitro analysis of amino acid levels in complex ...
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Standard PDF - Wiley Online Library
Aug 23, 2012 ... Altered concentrations of these metabolites and ions have been implicated in the ..... Muscle sampling and analysis. On the day of the ..... Automated analysis of cellular metabolites at nanomolar to micromolar concentrations ...
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Title: Performance and metabolism in repeated sprint exercise
Feb 18, 2008 ... muscle metabolites between the MI and LI trials suggest that any ..... Arthur PG, Hochachka PW (1995) Automated analysis of cellular metabolites at nanomolar to micromolar concentrations using bioluminescent methods.
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Intracellular ATP Concentration Contributes to the Cytotoxic - Plos
Oct 1, 2013 ... micromolar concentrations; and by interfering with the homeostasis of the intracellular ... design, data collection and analysis, decision to publish, or preparation of the manuscript. ... pathways: by activating cell surface receptors at nanomolar/ ... automatic microplate reader (Sunrise, Tecan Group Ltd.,.
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Metabolite profiling and pharmacokinetic evaluation - [email protected]
May 4, 2015 ... microphysiological hepatocyte-Kupffer-cell coculture system and .... dexamethasone, are used at nanomolar to micromolar concentrations in cultures of primary ..... processed using Agilent MassHunter qualitative analysis software ( v. B.06) ..... Towards automated production and drug sensitivity testing using ...
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Differential Proteome Analysis of Mouse Plasma Lipoproteins
Apr 2, 2014 ... Measurement of protein biomarkers involved in TG metabolism. ... At the cellular level, the synthesis and secretion of VLDL resembles that of ... apolipoproteins ( e.g., apoA1 and apoB100) in micromolar concentration range from only ..... ApoB48 and apoA5 plasma levels are at single-digit nano-molar range ...
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