Structural characterization by 13C-NMR spectroscopy of products
glycosyl donors: application to a UDP-glucose:(133)-b-D-glucan synthase from blackberry (Rubus ... Key words: 13C-NMR spectroscopy/in vitro synthesis of.
[ download?doi=10.1.1.334.5649&rep=rep1&type=pdf - Read/Download File
hplc and 13c nmr for the analysis of transamidation in dynamic
In dynamic combinatorial chemistry (DCC) an enzyme is used to control the ... reaction is complex, analytical procedures limit possible application of the method. In the ... 13C NMR for analysis of a DCC system. ... proaches, which attempt in-vitro selection, an isolated receptor controls ... 13C NMR spectra were simulated by.
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Kinetic Analysis of Dynamic 13C NMR Spectra: Metabolic - NCBI
13C NMR spectroscopy of tissue metabolism has been ap- plied to numerous living systems and has generated wide- spread interest for application to laboratory investigations of intact organs and in ... detected in in vitro spectra of tissue extracts from different ... been tested in intact tissues, although isolated enzyme mea-.
[ biophysj00055-0462.pdf - Read/Download File
applications of 13c nmr in the study of biosynthetic mechanism
periment, the application of 'H- and 3H-nmr spectroscopy has illuminated the course of certain key biosynthetic steps in ... tween X-ray diffraction and nmr analysis of biomolecules. ... first three enzymatic steps consist of successive C- methylations of uro'gen 111 at C-2 (2),. C-7 (31 .... moser's in vitro analogy (17). The final ...
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31P and 13C nuclear magnetic resonance studies of metabolic
in cell-free extracts in vitro using 31P NMR spectroscopy and using enzyme assays. The NMR data indicate .... 13C NMR analysis agreed with data obtained by enzymatic analysis. .... P. multocida . This method uses an enzymatic reaction.
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31PNMR and 13CNMR studies of mannose metabolism in
cell-free extracts in vitro using 31P-NMR spectroscopy, and by enzyme assays. Plesiomonas ... Investigation of mannose metabolism using in vivo 13C NMR showed mannose 6-phosphate accumulation ... The PTS uses phosphoenolpyruvate as the phos- .... and phosphoenolpyruvate were determined by NMR analysis.
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Model Free Approach to Kinetic Analysis of Real-Time - Plos
Sep 4, 2013 ... Magnetic resonance spectroscopy (MRS) is capable of distin- guishing endogenous ... imaging of enzyme kinetics in biological systems using 13C MRS. The most widely explored application of hyperpolarized 13C MRS to date is the ... We also apply the AUC analysis methods to in vivo data sets from mice.
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Nuclear Magnetic Resonance Evaluation of Metabolic and
Incontrast to changes in '3C NMR spectra, relative high-energy phosphate levels from 3`P spectra were similar at all ..... versus the C-3 glutamate signal within the in vitro 13C spectra as ..... vitro analysis of the enzymatic activities within the tricarboxylic .... applications to "3C NMR studies in perfused rat hearts. J Biol Chem.
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In vivo C NMR spectroscopy and metabolic modeling in the brain: a
In vivo 13C NMR spectroscopy has the unique capability to measure metabolic fluxes noninvasively in the brain. Quantitative measurements of metabolic fluxes require analysis of the 13C labeling time courses ... modeling which uses a one- compartment model and ... For example, the enzyme pyruvate carboxylase is.
[ Henry_MRI_Rev_2006.pdf - Read/Download File
Glutathione, but not glutamine, is detected in 13C-NMR spectra of
The analysis of the pH dependence of chemical shifts from various ... C6 cell; Glutathione; Glutamine; "C-NMR spectroscopy 1. .... proteins and DNA, enzyme turnover of GSH was probably lower than that of gluta- activity, .... I., tion of the metabolism of these cells in vitro, and more stem, J. and Nissim, I. (1990) J. Neurochem.
[ 1-s2.0-001457939381009O-main.pdf?_tid=6dd9efe4-b13e-11e3-97b3-00000aab0f02&acdnat=1395436924_b5d4456b85d3d4845193ef525d3ef384 - Read/Download File
Pyruvate Metabolism in Halobacterium salinarium Studied by
Intracellular 13C Nuclear Magnetic Resonance Spectroscopy. SUKESH R. BHAUMIK ... about the stability of the enzymes in vitro. 13C nuclear ... in vitro milieu in studies of metabolic pathways. The results of ..... Carbon-13 nuclear magnetic resonance analysis of ... NMR spectroscopy: new methods and applications. Ameri-.
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Running head: Cellulose analysis Name: Seth DeBolt Address
Mar 19, 2010 ... Tools for cellulose analysis in plant cell walls. Darby Harrisa .... 13C-NMR spectroscopy would not only confirm that the crystalline structure of ...
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13C NMR study of the effects of leptin treatment on kinetics of
treatment in vitro and markedly decreased hepatic de novo synthesis of fatty ... tocyte, 13C NMR spectroscopy helped identify the metabolic ... NMR Conditions and Analysis. 13C ..... by ACC, the key regulatory enzyme of de novo synthesis of .
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Full Text (PDF) - Proceedings of the National Academy of Sciences
Nov 11, 2008 ... discovered as a candidate by a two-phase in vitro polymerization ... substrate specificity CoA transferase enzyme engineering ... purpose applications, as described in numerous patents. ..... (B) 13C-NMR spectrum of a P(LA-co-3HB) copolymer isolated from the recombinant E. coli harboring the plasmid ...
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probing plant metabolism with nmr - UFV
Nuclear magnetic resonance (NMR) spectroscopy can be used to analyse the composition of tissues both in vivo and in various extracts. ... hampered by the low sensitivity of the 13C nucleus (which is four-fold .... enzymes of central metabolism (e.g. the activities of. ATPases ... physiological applications to plants. In Nuclear ...
[ NMR.pdf - Read/Download File
direct nuclear magnetic resonance spectroscopic analysis of 13c
by 100-MHz 13C NMR spectroscopy under the conditions of dis- tortionless enhancement ... variation of in vivo activities of the conjugation enzymes as well as oxidative ... troublesome to use and unsuitable for application to humans because.
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Download as a PDF - CiteSeerX
was confirmed by use of 13C NMR spectroscopy and gas chromatography–mass ... enzyme dimethylglycine dehydrogenase (DMGDH). A .... For the analysis of the NMR spectra, 1D ..... application of in vitro 1H NMR spectroscopy in diagnos-.
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Application of stable isotope labelling in cell culture experiments: [2
In particular, the incorporation of 13C-labels at specific carbon positions of a given metabolite can ... multinuclear NMR-spectroscopy to characterize the metabolism of labeled ... NMR analysis: Lyophilized samples were redissolved in 0.5 ml D2O. ... enzyme) as well as [3-13C]- and [2-13C]aspartate (conversion of labeled.
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Drosophila Larvae - The Journal of Biological Chemistry
... vitro by means of. 13C nuclear magnetic resonance spectroscopy. ... correlated with the in vitro alcohol dehydrogenase activities in ... enzyme system in Drosophila larvae can be a major target of ... We report here the application of 13C nuclear magnetic reso- .... Analysis of variance indicated that the variation among the.
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Astroglial Contribution to Brain Energy Metabolism in Humans
Revealed by 13C Nuclear Magnetic Resonance Spectroscopy: Elucidation of the ... The further application of this ap- ... been used in in vitro 13C NMR studies of animals (Kunnecke and .... converted back to glutamate by the enzyme phosphate-activated .... sessed from a detailed analysis of the time course of glutamate.
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