High-resolution Mass Spectrometry and Accurate Mass - Wiley
77843-3255, USA. Fundamental concepts of high-resolution mass spectrometry as it relates to the issue of accurate mass measure- ... mass analyzers equipped with gas chromatograph intro- .... definition, m/Dma ... low-resolution instruments, e.g., single-focusing or ..... analysis of proteins which have sample heterogeneity.
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Mass Spectrometry and Protein Analysis
Mass spectrometry is a central analytical technique for protein research and for the study of biomolecules in ... valent structure of single, highly purified proteins,.
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1. Mass spectrometry - Royal Society of Chemistry
Mass spectrometry in some form is familiar to many people whose chemistry ... If masses are required to one atomic mass unit (one dalton) a single focusing ..... known as the mean free path, and is of the order of several hundred metres when the ..... analysis the technique is destructive, and the sample cannot be recovered .
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An Introduction to Mass Spectrometry - Widener University
Sep 2, 1998 ... Electric Sector/Double Focusing Mass Spectrometers . .... that use direct ionization techniques to routinely analyze proteins ... M is the molecular ion produced by removing a single electron to form a radical cation. .... mean free path between collisions is only 10 meters and analyte molecules undergo many.
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Untitled-1 - Scripps Center for Metabolomics and Mass Spectrometry
a minimum amount of sample preparation has made the analysis of proteins and peptides routine.” Peptide ... Acronym | Means of Ionization electrospray ... Fortunately, electrospray mass spectrometers are equipped with computer ..... extract multiple molecular weight calculations from a single spectrum. Thus, one can ...
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Matrix-assisted Laser Desorption/Ionization Mass Spectrometry in
MALDI MASS SPECTROMETRY IN PEPTIDE AND PROTEIN ANALYSIS. 1. Matrix-assisted Laser .... DE is a relatively simple means of cooling the ions (and possibly focusing them) ..... globular domains separated by a single long central a- helix (amino acids 65–92). .... A MALDI mass spectrometer equipped with auto -.
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FTICR-mass spectrometry for high-resolution analysis in - RCI
spectrometric technique (a means of analysis of such compound libraries with ... brary; mass spectrometry; single bead analysis; re- view ... complex peptide libraries.8,9 Presently, mass spec- trometry ... equipped with three main components: a superconduc- tive magnet .... focusing as encountered in all other types of mass.
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Solution Isoelectric Focusing for Peptide Analysis - American
Jul 18, 2005 ... Separation of peptides by solution isoelectric focusing (sIEF) offers the ... tions and mass spectrometry instrumentation is evolving ... pared with a single dimension LC-MS analysis. .... equipped with an on-line microcapillary HPLC ( ThermoElec- .... means there are peptides re-identified between fractions.
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Thermo Scientific LTQ Orbitrap An Overview of the Scientific Literature
identification to the analysis of trace-level components in complex mixtures, be it in ... Acquiring tandem (MS/MS) mass spectra of peptides with a high mass .... The studies focusing on the proteomics of organelles have to deal with an .... be detected in a single experiment using the LTQ Orbitrap where the accuracy of the ...
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Detection of 25,000 molecules of substance P by MALDI-TOF mass
Mass spectrometric analysis of peptides with a total sample loading of several ... While single molecule analysis by mass spectrometry has not been ... on a home- built linear time-lag focusing MALDI-TOF mass spectrometer equipped ..... In theory, single ion detection with isotope-depleted samples can be used to define the ...
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Mass spectrometry for the quantification of bioactive peptides in
''absolute'' to define the scope of this review and to distinguish it from reports ... analysis would suggest that peptide quantification could ideally ... of bioactive peptides in biological fluids, and focus on those mass ..... A. LC-MS in Full Scan ( FS) or Single Ion .... mass spectrometer equipped with a TurboIonSpray interface for.
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Data-controlled automation of liquid chromatography/tandem mass
This technique directs the mass spectrometer data system to focus on the ... (San Jose, CA) TSQ-700 triple quadrupole mass spectrometer equipped with a ... ICL programs define the mass list as either a list of masses to select for CID analysis or a ..... The sequence of each peptide by using the single letter amino acid code ...
[ 1-s2.0-1044030596000578-main.pdf?_tid=0b3d6a72-dc4b-11e3-accc-00000aab0f6c&acdnat=1400170242_557a187272af1e6e2e55f936a402b4ef - Read/Download File
Visualization and analysis of molecular scanner peptide mass spectra
PMF compares the list of experimental masses of peptides, the peptide mass .... Applied Biosystems, Framingham MA), which was equipped with a 337 nm UV laser. ... VRML data files and m/z-software by Proteometrics to render single spectra. ... at 804.4 Da, 820.4 Da, 838.2 Da and 936.1 Da by means of signal intensity.
[ 1-s2.0-S1044030501003580-main.pdf?_tid=eec2a230-4d39-11e4-a37e-00000aab0f02&acdnat=1412587374_55c65c4519a39c64555294df6cd51d32 - Read/Download File
Optimization of a MALDI TOF-TOF mass spectrometer for intact
Recently, a MALDI TOF mass spectrometer with the novel “LIFT” technique ( MALDI ... were designed for tandem mass spectrometry analysis of tryptic peptides, we ... proteomics analyzer equipped with TOF/TOF ion optics and a diode pumped ... the collision gas such that nominally single collision conditions were achieved.
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Direct analysis of protein complexes using mass spectrometry
actions generally focus on one target gene or protein at a time4. Even ... The peptides are then eluted from the RP column into the mass spec- trometer using a gradient of ... Many of the protein identifications were based on single-peptide fragmentation ... be identified. Various 2D electrophoretic methods used to define the.
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Imaging mass spectrometry: fundamentals and applications to drug
Imaging mass spectrometry (IMS) encompasses a variety ... pounds present in biological tissues with at least single-cell .... Static SIMS: A mode of SIMS surface analysis using less than a total primary ion ... System optimized for the two- dimensional peptide MALDI imaging of cells or ... The instrument can be equipped with.
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1 Introduction to Mass Spectrometry, a Tutorial - Wiley-VCH
techniques and mass analyzers, the focus is on data interpretation and ... sample introduction, the mass spectrometer continuously acquires mass spectra, that is ... define resolution as m/Δm, where m is the mass of the ion (and obviously should ..... MALDI-TOF-MS is widely applied especially in the analysis of proteins, pep-.
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Standard PDF (745.2 KB) - Wiley Online Library
by using fast-atom-bombardmentltandem mass spectrometry ... Analysis of peptides can be achieved with sample sizes less than 0.1 pmol and be completed within ... means seems now to be feasible . ... eter (BEB configuration); the instrument is equipped with two ... The ion of interest was focused in the single focus with.
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Applications of Tandem Mass Spectrometry: From Structural - InTech
Feb 29, 2012 ... somewhat reductionist, definition of mass spectrometry. .... Tandem mass spectrometers in space are either double focusing mass ... (2), and it is equipped with a collision cell (3) making possible to perform CID .... Early mass spectrometers were capable of only one stage of mass analysis and structure.
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Oxidative Decarboxylation of Peptides Catalyzed by - The Journal of
Mass spectrometry was used to compare the kinetics of ... Sequence analysis of modiﬁed proepidermin ... Chemical Synthesis of Peptides and Peptide Libraries —Single pep- ... 2400 equipped with a nebulizer-assisted electrospray (“ion spray”) ... focusing was carried out according to the instruction manual of Serva.
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