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An Improved Procedure for Enzymatic Digestion of Polyvinylidene
An improved and simplified procedure for enzymatic digestion of proteins bound to polyvinylidene difluoride (PVDF) membranes for obtaining internal protein ...
[ 1-s2.0-S0003269784711481-main.pdf?_tid=2ca9f74a-9076-11e4-8958-00000aacb35f&acdnat=1419979975_0e0ae3cc2008e12f1fa978d70bdeb2b2 - Read/Download File

Actin binding protein fesselin.pdf - The ScholarShip at ECU - East
and transferred on Sequi-Blot™ PVDF Membrane (BIO-RAD) in a tank blot apparatus [18]. ... PVDF membrane [19]. ..... An improved procedure for enzymatic digestion of polyvinylidene difluoride-bound proteins for internal sequence analysis.
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Covalent Modification of Rat Liver Dipeptidyl Peptidase IV (CD26
Our previous study showed that diclofenac metabolites bound covalently .... the 110 kDa protein on PVDF (2 lanes of 20 pg/lane) was reduced in ... The digested protein fragments ..... (21) Fernandez, J., Andrews, L., and Mische, S. M. (1994) An improved procedure for enzymatic digestion of polyvinylidene difluoride- bound ...
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Purification and properties of an unusual NADPH-dependent ketose
All procedures were performed at 4°C. Twenty grams of frozen ..... An improved pro- cedure for enzymatic digestion of polyvinylidene difluoride-bound proteins ...
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Methodical Analysis of Protein-Nitrocellulose - ResearchGate
enzymatic digestion of NC-bound protein, in terms of ... dez procedure for digestion of PVDF-bound proteins in ..... The apparently improved elution ...
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Role of the Src homology 2 domains and interdomain regions in
ZAP-70 enzymatic activation is strictly dependent on the binding, via its ... motif; KD, kinase defective; PVDF, poly(vinylidene diflouride);. SH2, Src ...... Fernandez, J., Andrews, L. & Mische, S.M. (1994) An improved procedure for enzymatic digestion of polyvinylidene difluoride- bound proteins for internal sequence analysis.
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Purification and characterization of a cobalt‐activated
~Hardeman et al., 1998! and enzymatic digestion by carboxypep- tidases ~ Thiede et al., ... drophobic and gel filtration chromatographic procedures yielded. 11 mg PfuCP from 21 g ...... An improved procedure for enzymatic digestion of polyvinylidene difluoride-bound proteins for internal sequence analysis. Anal Biochem ...
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The Karyopherin Kap142p/Msn5p Mediates Nuclear Import and
Feb 19, 2001 ... yeast cytoplasm, proteins bound to Rpa2p-PrA were im- ..... in the process of DNA DSB repair requires more RPA .... An improved procedure for enzymatic digestion of polyvinylidene difluoride-bound proteins for internal.
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A Nuclear Import Pathway for a Protein Involved in tRNA Maturation
Dec 29, 1997 ... formed using published procedures (Aitchison et al., 1995). For protein A tagging, a PCR ..... An improved procedure for enzymatic digestion of polyvinylidene difluoride-bound proteins for internal sequence analysis. Anal.
[ 1655.full.pdf - Read/Download File

Structure - Andrej Sali Lab
Apr 11, 2006 ... An improved procedure for enzymatic digestion of polyvinylidene difluoride- bound proteins for internal sequence analysis. Anal. Biochem. 218,.
[ Dokudovskaya_Structure_2006.pdf - Read/Download File

A DNA-binding protein from Candida albicans - Microbiology
An improved procedure for enzymatic digestion of polyvinylidene difluoride- bound proteins for internal sequence analysis. Anal Biochem 218,. Freeman, K.
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Components of Coated Vesicles and Nuclear Pore Complexes
the NPCs and vesicle-coating complexes arose by a process of divergent ..... Sci 29: 175–182. Fernandez J, Andrews L, Mische SM (1994) An improved procedure for enzymatic digestion of polyvinylidene difluoride-bound proteins for.
[ 04_devos_plos-biology.pdf - Read/Download File

A Distinct Nuclear Import Pathway Used by - Rockefeller University
May 30, 1997 ... Laskey, 1991; Makkerh et al., 1996) are bound by karyo- pherin ...... improved procedure for enzymatic digestion of polyvinylidene difluoride-.
[ 34_1997_Cell_Rout.pdf - Read/Download File

Intra- and Extracellular β-Galactosidases from Bifidobacterium
BIF3, is most probably an extracellular enzyme, since it contained a signal sequence which was cleaved off .... Digestion of BIF3 polypeptide bound to PVDF with endoproteinase Lys-C and extraction of the resultant ..... An improved procedure.
[ 2276.full.pdf - Read/Download File

Purification of a Cytochrome bd Terminal Oxidase Encoded by the
Nov 9, 1995 ... Both enzymes can nonetheless generate a proton motive force ... standard procedures with a DNA library from B. firmus OF4 that had been ... fragment into XbaI-digested pGEM3Zf() resulted in the pMS101 .... PVDF-bound polypeptide, purification of the resulting peptides by ..... An improved procedure.
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Paleoproteomics explained to youngsters: how did the - Hal
Aug 6, 2014 ... PVDF membranes allowed the direct sequencing of N-terminally .... [4] Diezel W, Kopperschlager G, Hofmann E. An improved procedure for protein .... Analysis - Enzymatic Digestion for Less Than 10 Mu-G of Protein-Bound to.
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Calmodulin Modulates the Interaction between IQGAP1 and Cdc42
... membrane (PVDF). Protein Digestion—The procedure for digestion of proteins in poly- .... enzyme specificity of trypsin as a limiter, a search was made of. FIG. 2. Identification of ... was obtained by transferring to PVDF proteins that bound to ..... tially contribute to improved methods of prevention, detection and treatment of ...
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This paper is first published in Journal of Proteomics 2014 Jul 31
PVDF membranes allowed the direct sequencing of N-terminally free proteins .... [ 4] Diezel W, Kopperschlager G, Hofmann E. An improved procedure for protein .... Analysis - Enzymatic Digestion for Less Than 10 Mu-G of Protein-Bound to.
[ 1408.1325 - Read/Download File

Identification and cloning of GCA1, a gene that - Medical Mycology
Oct 5, 1998 ... Electrotransfer to a PVDF membrane (PRO-BLOTT, ..... An improved procedure for enzymatic digestion of polyvinylidiene difluoride-bound.
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Systematic Analysis of Peptide Recoveries from In-Gel - NCBI
results suggest an optimized in-gel trypsin digestion strategy in which proteins in ... stained gels or bound to PVDF membranes,7–10 and proteolysis of more ...
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