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An improved method of high‐voltage isoelectric focusing on
An improved method of high-voltage isoelectric focusing on cellulose acetate membranes. An apparatus and the procedure for cellulose acetate isoelectric ...
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Isoelectric Focusing Procedures for the Analysis of Allozymic
protein concentrating effects of isoelectric focusing (IEF) but differ in the type of support ... IEF in up to five layers of cellulose acetate membrane allows .... with breath vapor just before applying voltage. ..... An improved method of high- voltage ...
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Further observations on isoelectric focussing of serum proteins
Treatment of cellulose acetate gel strips by boron trifluoride in methanol has been ... the general isoelectric focussing method including improved electrode solutions. ... trifluoride in methanoi to make it suitable for low voltage isoelectric focussing. .... The high concentration of ampholytes m the form of a pH gradient following ...
[ 1-s2.0-000989817890150X-main.pdf?_tid=137555aa-396c-11e4-a3fd-00000aab0f01&acdnat=1410409887_88c27411e466cece63b0425db3878c60 - Read/Download File

A family with high serum leucine aminopeptidase activity derived
Patient and Methods ... The cellulose acetate membrane after isoelectric focusing ..... improved method of high-voltage isoelectric focusing on cellulose acetate ...
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AlkalinePhosphataselsoenzymesResolvedby - Clinical Chemistry
Elevated muscle creatune kinase MB ... Rosalki SB. An improved procedure for serumcreatine phos- ... With this electrophoretic method the liver, biliary, and bone ... cellulose acetate membranes (2) or polyacrylamide gels (3), isoelectric focusing (4), chemical inhibition (5), heat dens- .... mm at this voltage is the minimum.
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HemoglobinA1 byIsoelectricFocusing - Clinical Chemistry
fraction (HbA1; glycohemoglobin) measured in most methods have not been fully defined ... The greater specificity of HbA1 by isoelectric focusing makes it an ... System and a High Voltage ..... authors. (17) to 10-12#{176}C,which improved the sharpness of the ... a novel system of specially prepared cellulose acetate mem-.
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High-Performance Capillary Isoelectric Focusing with a
formance capillary lsoelectrk focuslng (IEF) performed In a. 12 cm long ... This technique is able to resolve proteins which ... by a high-voltage dc power supply ( Spellman, Plainview, NY). The current ... cellulose acetate filters (Sartorius, Gottingen, Germany). The ..... centration gradient detector can be improved by using a.
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Principles and Applications of Capillary Isoelectric Focusing
May 1, 2014 ... force field, a steep voltage gradient forcing this army of amphoteres to join arms in an ... Isoelectric focusing is a high-resolution electrophoretic technique for separation and ... the development of improved experimental procedures. ..... a hydrophilic polymer such as hydroxymethyl cellulose is added, which.
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Hemoglobinopathies: Current Practices for Screening, Confirmation
Cellulose Acetate Electrophoresis (Alkaline) ... Appendix A: Examples of Molecular Methods for Hb Mutation Detection ..... screening, such as Isoelectric focusing (IEF) and High performance liquid chromatography (HPLC) .... High voltage protein separation is performed ... There is improved detection of sickle cell disease.
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Characterisation of the mobility on isoelectric focusing of individual
Methods. ISOELECTRIC FOCUSING. This was carried out on LKB Ampholine ready prepared thin ... complexes in the cellulose acetate strips were compared ...
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Download Sample pages 2 PDF - Springer
separation of components of an analyzed sample owing to external voltage gen- ... Isotachophoresis, and Isoelectric Focusing (Fig. 2.1). .... Thus, a shorter capillary, higher voltage, and higher mobility make the migration time of ... The resolution can be improved by lengthening of the capillary, changing .... Cellulose acetate.
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Full Text (PDF)
May 20, 1985 ... This improved technique of isoelectric focusing was used to ... isoelectric focusing-a method for the separation of proteins ... small pH range produce notably higher resolutions of pro- ..... identical by cellulose acetate, starch gel,or polyacrylamide ... precluded the application of sufficiently high voltages that.
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200 years of Electrophoresis - Chromatography Today
Dec 6, 2010 ... the name of the technique should be changed to electrophoresis which is ... cellulose acetate and later nitrocellulose and. Ponceau S dye ... Cover of a Locarte of London High voltage electrophoresis catalogue of ... The isoelectric- focussing step was much improved with the introduction of immobilised pH ...
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Electrophoresis -
moving boundary technique for the electrophoretic separation of sub- stances, for which .... electrophoresis, isotachophoresis and isoelectric focusing. Even an ... high voltage ... been abandoned in profit of gel electrophoresis, because of improved ... Cellulose acetate membrane electrophoresis: cellulose acetate mem -.
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Separation of Bacterial Cells by Isoelectric Focusing, a New Method
Feb 15, 1996 ... A simple isoelectric focusing (IEF) method for whole bacterial cells .... (440 ml) was filtered through an 8-m-pore-size filter (cellulose .... agarose gels in 1 TAE buffer (40 mM Tris-acetate [pH 8.0], 1 mM EDTA). .... tant for the effect of high voltage. ... nificantly improved the molecular biological analysis of the.
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Sample Chapter - Wiley-VCH
High voltage power supply. 30000 V ... (IEF). Even an additional new method – a hybrid of electrophoresis and chro- matography .... doned in favor of gel electrophoresis because of the latter's improved separation and the higher ... and polyacrylamide gels are better, cellulose acetate membranes are more often replaced by ...
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Combining capillary electrophoresis with mass spectrometry for
Keywords: Capillary electrophoresis / Capillary isoelectric focusing / Capillary zone ... ionization method for CE-MS is electrospray ionization ..... vation by pumping a solution of cellulose acetate into an ..... established that higher CA concentrations improved the ..... CZE, using switching of applied voltages to control frac-.
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Biophysical & Biochemical Techniques
Isoelectric focusing ... in an electric field is one of the important methods of separation. .... Strip of paper or other fibrous material such as cellulose acetate .... Higher voltage gradients can be applied for better results if there is ... superseded other forms of electrophoresis because much improved resolutions are obtained and.
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Method of generating pH functions in electrophoresis and isoelectric
Jan 31, 1989 ... G. V. Troi'tsky et al., “Isoelectric Focusing of Proteins. Using a pH Gradient ... methods, and improved reagents suitable for generating ... An externally-applied voltage is used to drive the differ ..... where there is a higher concentration of polyhydroxyl .... solid form (such as paper or cellulose acetate), the poly.
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PZ-Peptidase from Chick Embryos - The Journal of Biological
acetate buffer, pH 5.0 (6 h with buffer change at 3 h). Approximately ... Digestion of Gelatin Peptides -A method was devel- oped to ... high voltage paper electrophoresis on Whatman ... DEAF-cellulose column in preparation for isoelectric focusing. (there was a .... Stability is improved by raising the ionic strength to 0.1 or by.
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