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Biochemical characterization of the human carbonic anhydrase
Some biochemical properties of a new red cell human carbonic anhydrase ... CA I isoenzyme, a proposal for the site of the amino acid substitution is presented. ... Purification of the variant enzyme was carried out using the affinity .... Osborne, W.R., Tashian, R. E.: An improved method for the purification of carbonic an-.
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Purification of carbonic anhydrase isoenzymes by high-performance
It is possible to purify carbonic anhydrases by affinity chromatography by coupling ... given by this method, does not exceed the depth of the active-site cleft [6]. ..... provided advantages due to shorter separation time and improved resolution.
[ 1-s2.0-0378434791802517-main.pdf?_tid=1692f01e-f990-11e4-9db0-00000aab0f01&acdnat=1431535977_9a2c3f7d8b892e688acc3caddd855ca3 - Read/Download File

Cross-reactions among carbonic anhydrases I, II, and III studied by
Cross-reactions among carbonic anhydrases (CAs) I, II, and III were studied using a ... and (3) five monoclonal antibodies prepared by the hybridoma technique using ... carbonic anhydrase isozymes immunological cross-reactions monoclonal .... An improved method for the purification of carbonic anhydrase isozymes by ...
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Purification and Characterization of Human Salivary Carbonic
expression of the salivary carbonic anhydrase isozyme. From these results we .... to p-aminomethyl benzenesulfonamide, prepared by the method of. Khalifah et al . (1977) ... hydrases I and I1 were purified with an affinity column by the procedure of ... ably improved the removal of nonspecifically bound proteins from the gel.
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Immunoelectron Microscopy of Carbonic Anhydrase Isozyme VI in
Carbonic anhydrase (CA) was purified from the saliva of pilocarpine-treated rats by inhibitor-affinity chromatogra- phy, and its localization in the rat ...... Osborne WRA, Zshian RE: An improved method for the purification of carbonic anhydrase  ...
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The effect of bile salts on carbonic anhydrase - Wiley Online Library
Three isoenzymes of human carbonic anhydrase. (HCA) have been ... an affinity chromatography method (12), were a gift from Dr. ..... An improved method for the purifi- ... Engberg P, Millquist E, Pohl G, Lindskog S. Purification and some.
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Standard PDF (669.6 KB) - Wiley Online Library
0 FEBS 1997. The complete sequence, expression in Escherichia coli, purification ... the active-site residues are identical to those found in the high- activity human isozyme 11. ... Keywords: carbonic anhydrase; Neisseria gonorrhoeae ; cloning; purification. .... at 280 nm (E,,,,) of native enzyme was estimated by the method.
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Full Text (PDF) - Proceedings of the National Academy of Sciences
membrane-associated enzyme purified from human tissues. We have determined ... Only one mammalian carbonic anhydrase isozyme is known .... inhibitors. MATERIALS AND METHODS .... refinement (36) and model building improved the quality of the maps .... region may account for the 15-fold lower affinity of many CA.
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Physiological and Molecular Biological - Plant Physiology
A single intracellular carbonic anhydrase (CA) was detected in air-grown and, ... inorganic carbon, whereas 2 mM acetazolamide had little effect on the affinity for dissolved ... ends method and indicated that the cDNA encodes 282 amino acids. ... the N-terminal amino acid sequence of the purified CA indicated that it ...
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Interaction of amide inhibitors with the active site of carbonic
ABSTRACT: Most carbonic anhydrase (CA) inhibitors bind at the active site metal and either are anions or are capable of ... The pH dependence of the affinity of carbonic anydrase isozyme I (CA .... according to the affinity chromatography method developed ..... Furthermore, sensitivity would be improved by addition of the.
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Structural Basis of Inhibitor Affinity to Variants of Human Carbonic
understand the structural basis of enzyme-inhibitor affinity, we now report the dissociation rate and ... understanding the behavior of carbonic anhydrase isozyme 111, which bears a wild-type F198 side .... 198 variants were purified from these cell extracts using ...... Fierke, C. A., & Hammes, G. G. (1994) Methods Enzymol.
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Structure, Function and Applications of Metal-Requiring Enzymes
May 17, 2010 ... Requiring Enzymes: Carbonic Anhydrase and epi- ... affinity between human carbonic anhydrase II and the cryptophane .... 2.2.2 Experimental Methods .... SDS-PAGE analysis of the purification of epi-isozizaene synthase. ... The X-ray crystal structures of several human CA isozymes have been solved to-.
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Protein and Peptide Purification Applications - Bio-Rad
Edwards PA et al., Improved methods for the solubilization and assay of hepatic ... Andre C et al., Purification of muscarinic acetylcholine receptors by affinity ... human carbonic anhydrase B, Biochemistry 16, 2241–2247 (1977) .... Morin LG, Improved separation of creatine kinase cardiac isoenzyme in serum by batch.
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inhibition of choline acetyltransferase by monoclonal antibodies
In the present study, we describe an improved affinity chro- matography method for the purification of CAT from rat brain and the properties of ..... carbonic anhydrase. FRACTION ..... multiple CAT isozymes have been detected by means of iso-.
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Optimization of a Novel Peptide Ligand Targeting Human Carbonic
May 31, 2012 ... Recently, a new peptide with affinity for human carbonic anhydrase IX (CaIX-P1) was ... their small size peptides have improved pharmacokinetic proper- ..... suppliers and were used without further purification. .... BxPC3 cells in 75 cm2 cell culture flasks using the Trizol method .... anhydrase isozymes.
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Carbonic Anhydrase and Heavy Metals - InTech
Mar 2, 2012 ... In general, there are three distinct groups of CA isozymes within the .... The affinity of carbonic anhydrase for zinc is in subpicomolar range, ... Escherichia coli and purified aerobically [Alber et al., 1996], while it ... (d10), the d7electron configuration of Co(II) is accessible to electronic spectroscopic methods.
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curriculum vitae - Researchers @ Brown
resolution analytical methods, such as high performance liquid .... Purification of Carbonic Anhydrase Isoenzymes by High Performance Affinity ... Hoffer, L., Schwinn, H., Biesert, L., Josic, Dj. (1995), Improved Virus Safety and Purity of a.
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Influence of the Zn(II) Cofactor on the Refolding of Bovine Carbonic
could lead to improved methods of analyzing proteins or to different uses of SDS- PAGE. ... carbonic anhydrase II (BCA, EC, which contains a Zn(II) cofactor, as a model protein for ..... We purified BCA by affinity chromatography using 4-(ami- ... chromatography to remove the isozyme containing Glu56. We prepared a ...
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Download as a PDF
INHIBITION OF CARBONIC ANHYDRASE ISOZYMES AND II ... Abstract: The title compound was prepared by an improved variant of the literature .... were those described by Lindskog's group,2 and enzymes were purified by affinity chromatography according to the method of Khalifah et al.3 Enzyme concentrations were ...
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then purified by affinity chromatography on cycloheptaamylose (HJORTH, ... described (BLOOR, MFJSLER and NIELSEN 1981); protein was determined by the Lowry method. ... Cyanogen bromide cleavage: The purified isozymes, 1-2 mg/ml, were ... One mg of human carbonic anhydrase I, whose N-terminus is blocked,.
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