Glucoamylase starch-binding domain of Aspergillus niger B1 - NCBI
Aspergillus niger B1 (CMI CC 324262) was cloned and expressed in Escherichia ..... Equilibrium adsorption isotherms were used to study the affinity of purified ...
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Adsorption of Amyloglucosidase from Aspergillus niger NRRL 3122
Jun 27, 2005 ... Amyloglucosidase enzyme was produced by Aspergillus niger ... Key words: amyloglucosidase, adsorption isotherm, kinetics adsorption, ion exchange resin, purification. ∗ ... characteristics of glucoamylases on ion exchange.
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Production and purification of a granular-starch-binding domain of
A domain of glucoamylase 1 from Aspergillus niger which binds to granular starch was .... Adsorption isotherms for Gl (o) and G1C ( ) to granular corn starch.
[ 1-s2.0-001457939081191P-main.pdf?_tid=54fde8ac-fc62-11e4-97cb-00000aab0f6b&acdnat=1431846179_7894b4563ec425a141f18666e139f362 - Read/Download File
Starch‐binding domain shuffling in Aspergillus niger glucoamylase
Aspergillus niger glucoamylase consists mainly of two forms, GAI (from the ..... finding that both constructs followed Langmuir adsorption isotherms, the.
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Standard PDF (418.7 KB) - Wiley Online Library
C-terminal SBD from Aspergillus niger glucoamylase;. (ii) CBM21 ..... Adsorption of purified SBD proteins to cornstarch: D123 ... sent the slope of each isotherm.
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Production and purification of a granular‐starch‐binding domain of
domain of glucoamylase 1 from Aspergillus niger. Nigel J. Belshaw and Gary ..... Adsorption isotherms for Gl (0) and GlC (*) to granular corn starch. Protein (O-3 ...
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Expression in Aspergillus niger of the Starch‐Binding Domain of
Glucoamylase 1 from Aspergillus niger is an economically important enzyme in many industrial processes. ..... Binding isotherms for the interaction of the proteolytic (0) .... Chen, L., Ford, C. & Nikolov, Z. (1991) Adsorption to starch of a p-.
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Standard PDF (619.7 KB) - Wiley Online Library
model was fit to the binding isotherms, where P is protein,. S is starch, and Bmax is the .... Aspergillus niger glucoamylase fused to a b-galactosi- dase using corn ...
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Starchbinding domains in the CBM45 family lowaffinity - ETH Zürich
Affinities for starch and soluble cyclodextrin starch mimics were measured by adsorption assays, ..... domain from Aspergillus niger glucoamylase, binding site 2, which is .... bottom panel depicts the binding isotherm; open circles represent.
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Starch-Binding Domain Affects Catalysis in Two Lactobacillus
Aug 25, 2004 ... lated Gp-I domain of glucoamylase I from Aspergillus niger involved in ... adsorption onto raw starch granules, and secretion (7, 14, 15,. 23, 27, 38). ..... ear adsorption isotherms indicate the apparent equilibrium distribu-.
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Alpha-Amylase Starch Binding Domains - Applied and
Nov 10, 2006 ... Protein binding assays showed an increased capacity of adsorption as a function of the ..... Adsorption isotherms (tandem units) for CBM26 SBD. .... granular-starch -binding domain of glucoamylase 1 from Aspergillus niger.
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Functional demonstrations of starch binding domains - Springer
In addition, adsorption assays showed that OsttaSSIII‑A D2 ..... each isotherm ..... Aspergillus niger glucoamylase are essential for inducing a conformational.
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Separation of Amyloglucosidase Using β-Cyclodextrin/Chitosan
ration from Aspergillus niger on the P-cyclodextrin-chitosan system were studied. The effects of ... It was shown that the adsorption isotherm for this system ... adsorption-desorption characteristics of glucoamylases on cyclodextrin- immobilized ...
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Low cost single-step purification of endoglucanase from Aspergillus
isotherm at 4 °C showed maximum adsorption of enzyme at pH 5.0, which was in the ... Keywords: Adsorbing column, Aspergillus fumigatus, Gel electrophoresis, Langmuir adsorption isotherm, Single-step ..... characterization of a thermostable glucoamylase from ... purification and characterization of the Aspergillus niger.
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Role of the linker region in the expression of Rhizopus oryzae
Aspergillus niger glucoamylase. Ro GA. Rhizopus oryzae glucoamylase. CBM. carbohydrate-binding module. SBD. starch-binding domain ...
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Bacteroides multi-domain starch-binding proteins 1
Aug 21, 2012 ... The SusE-C only with GM3M3 isotherm was indicative of two ... Adsorption depletion assay—The affinity of purified ...... granules is enhanced by fusion of a starch binding domain from Aspergillus niger glucoamylase. Biochim.
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catalysis by enzymes immobilizedon tuned - Dyuthi - cusat
May 20, 2013 ... In this work, Aspergillus niger glucoamylase, Bovine liver catalase, Candida rugosa lipase were ... onto support by adsorption and covalent binding. .... 2.10 Enzyme adsorption isotherms --------------------------------------42.
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Improved Stabilities of Immobilized Glucoamylase on Functionalized
size, high adsorption capacity, thermal and me- ... rous materials yield a type IV adsorption isotherm ... solution, Sigma Aldrich) and Aspergillus niger glu-.
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Cationic polymer enhanced hydrolysis of cornstarch for the
101. 4.4.2. Cornstarch hydrolysis by glucoamylase with C-PAM. 104. 4.4.3. ..... Figure 25: Adsorption isotherm of 40% cationicity C-PAM (20 ppm) to starch. ... 60 ..... commercially are generally extracted from fungal strains Aspergillus niger or.
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Hydrolysis of softwood by Aspergillus mannanase: Role of a
Apr 15, 2011 ... of Aspergillus aculeatus VN was expressed in Aspergillus niger D15#26 using .... glucoamylase signal peptide (SglaA) was used to target protein ..... Adsorption isotherms of rec-ManCBM on Avicel cellulose and softwood. (△).
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