A technique of decalcification suited to electron microscopy of
A Technique of Decalcification Suited to. Electron Microscopy of Tissues Closely. Associated with Bone '. IRWIN L. BAIRD,' WILLIAM B. WINBORN AND DALE E.
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Microwave-Induced Fast Decalcification of Rat Bone for Electron
Braz Dent J 18(2) 2007. Microwave-induced bone decalcification for electron microscopy ... conventional method to 48 hours in the microwave-aided method. Key Words: ..... tion suited to electron microscopy of tissues closely associ- ated with ...
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Decalcification by perfusion. A new method for rapid - Digitum
electron microscope. For comparison, a conventional immersion technique required 72 hours of decalcification to accomplish ..... A technique of decalcification suited to electron rnicroscopy of tissues closely associated with bone. Anat. Rec.
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Techniques in Electron Microscopy of Animal Tissue - Veterinary
Oct 10, 2013 ... Standard protocol for tissue in transmission electron microscopy: ... Cytoplasm: plasma membranes of adjacent hepatocytes (bottom) are closely apposed and ... decalcification with OsO4 fixation, and increasing the time of.
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The rate of calcium extraction during EDTA decalcification from thin
Apr 28, 1980 ... Baird IL, Winborn WB, Bockman DE (1967) A technique of decalcification suited to electron microscopy of tissues closely associated with bone.
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Bone formation at a ceramic implant interface | SpringerLink
Baird, I.L., Winborn, W.B., Bockman, D.E.: A technique of decalcification suited to electron microscopy of tissues closely associated with bone. Anat. Rec.159 ...
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Preparation Techniques of Luminal and Hard Tissues for Scanning
The use of Scanning Electron Microscopy (SEM) in life sciences has ... Keywords: SEM; techniques; luminal organs; hard tissues. 1. ... generated by a suitable source, typically a tungsten filament or a field .... Compact bone consists of closely packed .... Decalcification is a process of removal of calcium salt from mineralized ...
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Cells, Tissues & Organs: Methods of Study
Cells, Tissues & Organs: Methods of Study. 1. LEARNING ... Decalcification. J. Artifacts. 1. .... or EM) results from coupling the probes to colored or electron- opaque reaction products .... interactions between the closely adjacent, abundant dye molecules .... adapted for electron microscopy (Saltpeter M J Cell Biol. 32: 379-389 ...
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Tissue Procurement, Processing, and Staining Techniques - Assets
technique and tissue processing on the final results that ... ogy, or electron microscopy—all of which are greatly af- ..... a suitable slide is obtained for microscopic examination; ..... that acid decalcification may incur. .... closely. Overheating the wax will cause unwanted thermal artifacts in the tissue and compromise its cellular ...
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Electron Microscopic Observations of the Nucleus, Glial Dome, and
Electron Microscopic Observations of the Nucleus,. Glial Dome ..... 1967 A technique of decalcification suited to electron microscopy of tissues closely associ-.
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Calcified tissue histochemistry: from microstructures to nanoparticles
techniques which permit the qualitative and quanti- tative analysis of ... very closely connected with its organic components. Matrices of ... areas of calcification (Bonucci et al., 1992) and, ... the electron microscope (De Bernard et al., 1986;.
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Expedited Bone Throughput Using Microwave Decalcification
Oct 6, 2010 ... manual method of decalcifying bones is one of the most time-consuming steps ... bone decalcification without compromising tissue quality. .... buffered saline (PBS ) (Electron Microscopy Sciences, ... container suitable for freezing samples (small metal bowl ..... Work closely with director of surgical pathology.
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fixation of neural tissues for electron microscopy by perfusion with
This paper describes in detail a method for obtaining nearly uniform fixation of the nervous ... at the electron microscopic level to an extent that has not been possible heretofore. ... after a suitable interval the tissue is excised and ...... In a study of decalcification of the liver by in vivo ... The numerous, closely packed, polyg-.
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Detection of Microecological Phenomena in Filled Teeth I
Jul 22, 1989 ... methods including light microscopy, transmission electron microscopy and scanning ... MATERIALS AND METHODS ..... technique of decalcification suited to electron. 3. ... microscopy of tissues closely associated with bone.
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A new method for imaging and 3D reconstruction of mammalian
microscopic integrity of the more delicate cochlear tissues. ... The tissue remains suited to ... plied to combined confocal and transmission electron mi- ... If decalcification of the cochlea is required (e.g. ..... closely aligned to the axis of focus.
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The collagen fibril organization in human articularcartilage - NCBI
Apr 15, 1976 ... network adapted to load-bearing at the joint. ... In the present study a new technique has been used which selectively ... short decalcification with EDTA exposed collagen fibrils in the ... Specimens of tissue were cut into strips approximately ... scanning electron microscopy consisted entirely of collagen.
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diseases of molluscs general information - OIE
Mar 2, 2016 ... While this is suitable for, for example, ... intact to the laboratory or fixed for subsequent decalcification, ... methods can be used in addition to histology, among which, tissue ... being used, in addition to electron microscopy, for specific ... agents belonging to genera encompassing closely related species.
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2007 Romano et al. exoskeleton of Homarus americanus as a
Lobster cuticle is a good example of this and a suitable model for studying ... americanus) was investigated using scanning electron microscopy. ... fact, skeletal tissues are fiber-reinforced composites where .... method is traditionally used to purify chitin for commercial ... 2d) closely resembles that of decalcified cuticle, but.
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View/Open - Lirias
were removed from the skull and decalcified with 4%. EDTA in distilled water .... w x1 I.L. Baird, W.B. Winborn, D.E. Bockman, A technique of decalcifi- cation suited for electron microscopy of tissues closely associated. Ž . with bone, Anat. Rec.
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Histology Lab Manual - Histology Laboratory Manual - Columbia
and eosin (H&E) stains various components of cells and tissues. 2. ... Note: There is a more complete description of methods for preparation of histological .... magnification (e.g., electron microscopy) is usually required to resolve the basal lamina. ..... (1) Bone may be decalcified by acid solutions prior to embedding and.
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